6XTX
CryoEM structure of human CMG bound to ATPgammaS and DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | 2 | DNA replication licensing factor MCM2 | polymer | 904 | 102034.1 | 1 | UniProt (P49736) Pfam (PF12619) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) In PDB | Homo sapiens (Human) | Minichromosome maintenance protein 2 homolog,Nuclear protein BM28 |
| 2 | 3 | DNA replication licensing factor MCM3 | polymer | 853 | 96043.3 | 1 | UniProt (P25205) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) In PDB | Homo sapiens (Human) | DNA polymerase alpha holoenzyme-associated protein P1,P1-MCM3,RLF subunit beta,p102 |
| 3 | 4 | DNA replication licensing factor MCM4 | polymer | 883 | 99119.5 | 1 | UniProt (P33991) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) In PDB | Homo sapiens (Human) | CDC21 homolog,P1-CDC21 |
| 4 | 5 | DNA replication licensing factor MCM5 | polymer | 734 | 82406.6 | 1 | UniProt (P33992) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21933) In PDB | Homo sapiens (Human) | CDC46 homolog,P1-CDC46 |
| 5 | 6 | DNA replication licensing factor MCM6 | polymer | 821 | 93010.3 | 1 | UniProt (Q14566) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF18263) In PDB | Homo sapiens (Human) | p105MCM |
| 6 | 7 | DNA replication licensing factor MCM7 | polymer | 719 | 81411.9 | 1 | UniProt (P33993) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) In PDB | Homo sapiens (Human) | CDC47 homolog,P1.1-MCM3 |
| 7 | A | DNA replication complex GINS protein PSF1 | polymer | 196 | 23022.5 | 1 | UniProt (Q14691) Pfam (PF05916) In PDB | Homo sapiens (Human) | GINS complex subunit 1 |
| 8 | B | DNA replication complex GINS protein PSF2 | polymer | 222 | 25336.8 | 1 | UniProt (Q9Y248) Pfam (PF05916) In PDB | Homo sapiens (Human) | GINS complex subunit 2 |
| 9 | C | DNA replication complex GINS protein PSF3 | polymer | 216 | 24562.6 | 1 | UniProt (Q9BRX5) Pfam (PF22466) Pfam (PF05916) In PDB | Homo sapiens (Human) | GINS complex subunit 3 |
| 10 | D | DNA replication complex GINS protein SLD5 | polymer | 223 | 26081.9 | 1 | UniProt (Q9BRT9) Pfam (PF05916) Pfam (PF16922) In PDB | Homo sapiens (Human) | GINS complex subunit 4 |
| 11 | E | Cell division control protein 45 homolog | polymer | 566 | 65650.6 | 1 | UniProt (O75419) Pfam (PF02724) In PDB | Homo sapiens (Human) | PORC-PI-1 |
| 12 | M | DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') | polymer | 70 | 21390.6 | 1 | synthetic construct | ||
| 13 | 2, 4, 5, 6, 7 | ZINC ION | non-polymer | 65.4 | 5 | Chemie (ZN) | |||
| 14 | 2, 3 | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 2 | Chemie (ADP) | |||
| 15 | 4, 7 | PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER | non-polymer | 523.2 | 3 | Chemie (AGS) | |||
| 16 | 4, 6, 7 | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) |
Sequence modifications
4: 1 - 863 (UniProt: P33991)
B: 1 - 185 (UniProt: Q9Y248)
E: 1 - 566 (UniProt: O75419)
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | initiating methionine |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Glu -10 | - | expression tag |
| Asn -9 | - | expression tag |
| Leu -8 | - | expression tag |
| Tyr -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Ser -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Thr 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Glu 186 | - | expression tag |
| Asn 187 | - | expression tag |
| Leu 188 | - | expression tag |
| Tyr 189 | - | expression tag |
| Phe 190 | - | expression tag |
| Gln 191 | - | expression tag |
| Gly 192 | - | expression tag |
| Ser 193 | - | expression tag |
| Ala 194 | - | expression tag |
| Trp 195 | - | expression tag |
| Ser 196 | - | expression tag |
| His 197 | - | expression tag |
| Pro 198 | - | expression tag |
| Gln 199 | - | expression tag |
| Phe 200 | - | expression tag |
| Glu 201 | - | expression tag |
| Lys 202 | - | expression tag |
| Gly 203 | - | expression tag |
| Gly 204 | - | expression tag |
| Gly 205 | - | expression tag |
| Ser 206 | - | expression tag |
| Gly 207 | - | expression tag |
| Gly 208 | - | expression tag |
| Gly 209 | - | expression tag |
| Ser 210 | - | expression tag |
| Gly 211 | - | expression tag |
| Gly 212 | - | expression tag |
| Ser 213 | - | expression tag |
| Ala 214 | - | expression tag |
| Trp 215 | - | expression tag |
| Ser 216 | - | expression tag |
| His 217 | - | expression tag |
| Pro 218 | - | expression tag |
| Gln 219 | - | expression tag |
| Phe 220 | - | expression tag |
| Glu 221 | - | expression tag |
| Lys 222 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gln 346 | Glu 346 | variant |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 740070.6 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 2824.1 | |
| All* | Total formula weight | 742894.7 |
