6XTX

CryoEM structure of human CMG bound to ATPgammaS and DNA

Functional Information from GO Data

Chain	GOid	namespace	contents
2	0000727	biological_process	double-strand break repair via break-induced replication
2	0000781	cellular_component	chromosome, telomeric region
2	0000785	cellular_component	chromatin
2	0003677	molecular_function	DNA binding
2	0003688	molecular_function	DNA replication origin binding
2	0003697	molecular_function	single-stranded DNA binding
2	0004386	molecular_function	helicase activity
2	0005515	molecular_function	protein binding
2	0005524	molecular_function	ATP binding
2	0005634	cellular_component	nucleus
2	0005654	cellular_component	nucleoplasm
2	0005664	cellular_component	nuclear origin of replication recognition complex
2	0005694	cellular_component	chromosome
2	0005730	cellular_component	nucleolus
2	0005737	cellular_component	cytoplasm
2	0006259	biological_process	DNA metabolic process
2	0006260	biological_process	DNA replication
2	0006268	biological_process	DNA unwinding involved in DNA replication
2	0006270	biological_process	DNA replication initiation
2	0006334	biological_process	nucleosome assembly
2	0006915	biological_process	apoptotic process
2	0016787	molecular_function	hydrolase activity
2	0016887	molecular_function	ATP hydrolysis activity
2	0017116	molecular_function	single-stranded DNA helicase activity
2	0019899	molecular_function	enzyme binding
2	0030174	biological_process	regulation of DNA-templated DNA replication initiation
2	0032508	biological_process	DNA duplex unwinding
2	0042393	molecular_function	histone binding
2	0042555	cellular_component	MCM complex
2	0043138	molecular_function	3'-5' DNA helicase activity
2	0046872	molecular_function	metal ion binding
2	0071162	cellular_component	CMG complex
2	0071353	biological_process	cellular response to interleukin-4
2	0090102	biological_process	cochlea development
2	1902975	biological_process	mitotic DNA replication initiation
2	1905775	biological_process	negative regulation of DNA helicase activity
3	0000727	biological_process	double-strand break repair via break-induced replication
3	0000781	cellular_component	chromosome, telomeric region
3	0003677	molecular_function	DNA binding
3	0003697	molecular_function	single-stranded DNA binding
3	0004386	molecular_function	helicase activity
3	0005515	molecular_function	protein binding
3	0005524	molecular_function	ATP binding
3	0005634	cellular_component	nucleus
3	0005654	cellular_component	nucleoplasm
3	0005658	cellular_component	alpha DNA polymerase:primase complex
3	0005694	cellular_component	chromosome
3	0005730	cellular_component	nucleolus
3	0005737	cellular_component	cytoplasm
3	0005813	cellular_component	centrosome
3	0006259	biological_process	DNA metabolic process
3	0006260	biological_process	DNA replication
3	0006268	biological_process	DNA unwinding involved in DNA replication
3	0006270	biological_process	DNA replication initiation
3	0006271	biological_process	DNA strand elongation involved in DNA replication
3	0016020	cellular_component	membrane
3	0016787	molecular_function	hydrolase activity
3	0016887	molecular_function	ATP hydrolysis activity
3	0017116	molecular_function	single-stranded DNA helicase activity
3	0030174	biological_process	regulation of DNA-templated DNA replication initiation
3	0032508	biological_process	DNA duplex unwinding
3	0042555	cellular_component	MCM complex
3	0048471	cellular_component	perinuclear region of cytoplasm
3	0071162	cellular_component	CMG complex
3	1902975	biological_process	mitotic DNA replication initiation
4	0000727	biological_process	double-strand break repair via break-induced replication
4	0000781	cellular_component	chromosome, telomeric region
4	0003677	molecular_function	DNA binding
4	0003678	molecular_function	DNA helicase activity
4	0003697	molecular_function	single-stranded DNA binding
4	0004386	molecular_function	helicase activity
4	0005515	molecular_function	protein binding
4	0005524	molecular_function	ATP binding
4	0005634	cellular_component	nucleus
4	0005654	cellular_component	nucleoplasm
4	0005694	cellular_component	chromosome
4	0006259	biological_process	DNA metabolic process
4	0006260	biological_process	DNA replication
4	0006268	biological_process	DNA unwinding involved in DNA replication
4	0006270	biological_process	DNA replication initiation
4	0006271	biological_process	DNA strand elongation involved in DNA replication
4	0016020	cellular_component	membrane
4	0016787	molecular_function	hydrolase activity
4	0016887	molecular_function	ATP hydrolysis activity
4	0017116	molecular_function	single-stranded DNA helicase activity
4	0030174	biological_process	regulation of DNA-templated DNA replication initiation
4	0032508	biological_process	DNA duplex unwinding
4	0042555	cellular_component	MCM complex
4	0071162	cellular_component	CMG complex
4	1902975	biological_process	mitotic DNA replication initiation
5	0000727	biological_process	double-strand break repair via break-induced replication
5	0000781	cellular_component	chromosome, telomeric region
5	0003677	molecular_function	DNA binding
5	0003688	molecular_function	DNA replication origin binding
5	0003697	molecular_function	single-stranded DNA binding
5	0004386	molecular_function	helicase activity
5	0005515	molecular_function	protein binding
5	0005524	molecular_function	ATP binding
5	0005634	cellular_component	nucleus
5	0005654	cellular_component	nucleoplasm
5	0005694	cellular_component	chromosome
5	0006260	biological_process	DNA replication
5	0006268	biological_process	DNA unwinding involved in DNA replication
5	0006270	biological_process	DNA replication initiation
5	0016020	cellular_component	membrane
5	0016787	molecular_function	hydrolase activity
5	0016887	molecular_function	ATP hydrolysis activity
5	0017116	molecular_function	single-stranded DNA helicase activity
5	0030174	biological_process	regulation of DNA-templated DNA replication initiation
5	0032508	biological_process	DNA duplex unwinding
5	0032991	cellular_component	protein-containing complex
5	0042555	cellular_component	MCM complex
5	0043138	molecular_function	3'-5' DNA helicase activity
5	0043229	cellular_component	intracellular organelle
5	0071162	cellular_component	CMG complex
6	0000727	biological_process	double-strand break repair via break-induced replication
6	0000781	cellular_component	chromosome, telomeric region
6	0003677	molecular_function	DNA binding
6	0003678	molecular_function	DNA helicase activity
6	0003697	molecular_function	single-stranded DNA binding
6	0004386	molecular_function	helicase activity
6	0005515	molecular_function	protein binding
6	0005524	molecular_function	ATP binding
6	0005634	cellular_component	nucleus
6	0005654	cellular_component	nucleoplasm
6	0005694	cellular_component	chromosome
6	0006259	biological_process	DNA metabolic process
6	0006260	biological_process	DNA replication
6	0006268	biological_process	DNA unwinding involved in DNA replication
6	0006270	biological_process	DNA replication initiation
6	0016787	molecular_function	hydrolase activity
6	0016887	molecular_function	ATP hydrolysis activity
6	0030174	biological_process	regulation of DNA-templated DNA replication initiation
6	0032508	biological_process	DNA duplex unwinding
6	0042555	cellular_component	MCM complex
6	0042802	molecular_function	identical protein binding
6	0071162	cellular_component	CMG complex
6	1902969	biological_process	mitotic DNA replication
6	1990518	molecular_function	single-stranded 3'-5' DNA helicase activity
7	0000727	biological_process	double-strand break repair via break-induced replication
7	0000781	cellular_component	chromosome, telomeric region
7	0000785	cellular_component	chromatin
7	0003677	molecular_function	DNA binding
7	0003678	molecular_function	DNA helicase activity
7	0003697	molecular_function	single-stranded DNA binding
7	0004386	molecular_function	helicase activity
7	0005515	molecular_function	protein binding
7	0005524	molecular_function	ATP binding
7	0005634	cellular_component	nucleus
7	0005654	cellular_component	nucleoplasm
7	0005694	cellular_component	chromosome
7	0005730	cellular_component	nucleolus
7	0005829	cellular_component	cytosol
7	0006259	biological_process	DNA metabolic process
7	0006260	biological_process	DNA replication
7	0006268	biological_process	DNA unwinding involved in DNA replication
7	0006270	biological_process	DNA replication initiation
7	0006271	biological_process	DNA strand elongation involved in DNA replication
7	0006974	biological_process	DNA damage response
7	0008283	biological_process	cell population proliferation
7	0016020	cellular_component	membrane
7	0016787	molecular_function	hydrolase activity
7	0016887	molecular_function	ATP hydrolysis activity
7	0017116	molecular_function	single-stranded DNA helicase activity
7	0030174	biological_process	regulation of DNA-templated DNA replication initiation
7	0032508	biological_process	DNA duplex unwinding
7	0042325	biological_process	regulation of phosphorylation
7	0042555	cellular_component	MCM complex
7	0043229	cellular_component	intracellular organelle
7	0071162	cellular_component	CMG complex
7	0071466	biological_process	cellular response to xenobiotic stimulus
A	0000811	cellular_component	GINS complex
A	0001833	biological_process	inner cell mass cell proliferation
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005694	cellular_component	chromosome
A	0005737	cellular_component	cytoplasm
A	0006260	biological_process	DNA replication
A	0006268	biological_process	DNA unwinding involved in DNA replication
A	0071162	cellular_component	CMG complex
A	1902983	biological_process	DNA strand elongation involved in mitotic DNA replication
B	0000228	cellular_component	nuclear chromosome
B	0000727	biological_process	double-strand break repair via break-induced replication
B	0000811	cellular_component	GINS complex
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005694	cellular_component	chromosome
B	0006260	biological_process	DNA replication
B	0006261	biological_process	DNA-templated DNA replication
B	0006268	biological_process	DNA unwinding involved in DNA replication
B	0032993	cellular_component	protein-DNA complex
B	0071162	cellular_component	CMG complex
C	0000811	cellular_component	GINS complex
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005694	cellular_component	chromosome
C	0006260	biological_process	DNA replication
C	0006268	biological_process	DNA unwinding involved in DNA replication
C	0071162	cellular_component	CMG complex
C	1902975	biological_process	mitotic DNA replication initiation
D	0000727	biological_process	double-strand break repair via break-induced replication
D	0000811	cellular_component	GINS complex
D	0001833	biological_process	inner cell mass cell proliferation
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005694	cellular_component	chromosome
D	0005737	cellular_component	cytoplasm
D	0006260	biological_process	DNA replication
D	0006261	biological_process	DNA-templated DNA replication
D	0006268	biological_process	DNA unwinding involved in DNA replication
D	0071162	cellular_component	CMG complex
E	0000076	biological_process	DNA replication checkpoint signaling
E	0000727	biological_process	double-strand break repair via break-induced replication
E	0003682	molecular_function	chromatin binding
E	0003688	molecular_function	DNA replication origin binding
E	0003697	molecular_function	single-stranded DNA binding
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005694	cellular_component	chromosome
E	0005813	cellular_component	centrosome
E	0006260	biological_process	DNA replication
E	0006268	biological_process	DNA unwinding involved in DNA replication
E	0006270	biological_process	DNA replication initiation
E	0031261	cellular_component	DNA replication preinitiation complex
E	0036064	cellular_component	ciliary basal body
E	0071162	cellular_component	CMG complex
E	1902977	biological_process	mitotic DNA replication preinitiation complex assembly

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue ZN 2 1000

Chain	Residue
2	CYS329
2	CYS332
2	CYS352
2	CYS355

---

site_id	AC2
Number of Residues	10
Details	binding site for residue ADP 2 1001

Chain	Residue
2	GLN531
2	LEU675
6	VAL618
6	ARG619
6	GLU622
2	PRO525
2	GLY526
2	ALA528
2	LYS529
2	SER530

---

site_id	AC3
Number of Residues	10
Details	binding site for residue ADP 3 1001

Chain	Residue
3	SER351
3	HIS353
3	SER393
3	VAL394
3	ALA395
3	LYS396
3	SER397
5	ARG371
5	GLU463
5	GLU614

---

site_id	AC4
Number of Residues	5
Details	binding site for residue ZN 4 901

Chain	Residue
4	CYS306
4	CYS309
4	CYS328
4	CYS331
4	SER336

---

site_id	AC5
Number of Residues	13
Details	binding site for residue AGS 4 902

Chain	Residue
4	TYR471
4	PRO512
4	GLY513
4	SER515
4	LYS516
4	SER517
4	GLN518
4	ASN618
4	MG903
7	MET369
7	ARG514
7	ARG604
7	LEU607

---

site_id	AC6
Number of Residues	2
Details	binding site for residue MG 4 903

Chain	Residue
4	SER517
4	AGS902

---

site_id	AC7
Number of Residues	13
Details	binding site for residue AGS 4 904

Chain	Residue
4	ARG497
4	ARG643
4	ARG732
4	GLU735
6	HIS359
6	SER399
6	THR400
6	ALA401
6	LYS402
6	SER403
6	ASN504
6	ILE548
6	MG902

---

site_id	AC8
Number of Residues	4
Details	binding site for residue ZN 5 1000

Chain	Residue
5	CYS172
5	CYS175
5	CYS197
5	CYS207

---

site_id	AC9
Number of Residues	5
Details	binding site for residue ZN 6 901

Chain	Residue
6	CYS158
6	ASP160
6	CYS161
6	CYS180
6	CYS185

---

site_id	AD1
Number of Residues	2
Details	binding site for residue MG 6 902

Chain	Residue
4	AGS904
6	SER403

---

site_id	AD2
Number of Residues	4
Details	binding site for residue ZN 7 1000

Chain	Residue
7	CYS184
7	CYS187
7	CYS206
7	CYS211

---

site_id	AD3
Number of Residues	17
Details	binding site for residue AGS 7 1001

Chain	Residue
3	ARG523
3	ALA660
3	ARG661
3	GLU664
7	GLU343
7	ILE344
7	TYR345
7	PRO383
7	GLY384
7	VAL385
7	ALA386
7	LYS387
7	SER388
7	GLN389
7	ASN489
7	LEU533
7	MG1002

---

site_id	AD4
Number of Residues	3
Details	binding site for residue MG 7 1002

Chain	Residue
3	GLN473
7	SER388
7	AGS1001

---

Functional Information from PROSITE/UniProt

site_id	PS00847
Number of Residues	9
Details	MCM_1 MCM family signature. GVCCIDEFD

Chain	Residue	Details
7	GLY440-ASP448
3	GLY449-ASP457
4	GLY569-ASP577
2	GLY582-ASP590
5	GLY440-ASP448
6	GLY455-ASP463

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:18669648

Chain	Residue	Details
E	TYR130
7	GLY384
7	ALA386
7	LYS387
7	SER388
7	ASN489
7	ARG514
7	ARG604
6	GLU622

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648

Chain	Residue	Details
E	SER144
E	SER148

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692

Chain	Residue	Details
D	SER12
D	SER16

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
B	LYS109
7	SER678
5	LYS696

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:20068231

Chain	Residue	Details
7	SER365
6	SER762

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163

Chain	Residue	Details
7	SER500

---

site_id	SWS_FT_FI7
Number of Residues	3
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
7	LYS15
4	SER34
7	LYS28

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P97311

Chain	Residue	Details
6	SER689

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:17525332

Chain	Residue	Details
6	THR791
4	THR110

---

site_id	SWS_FT_FI10
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
4	SER105

---

site_id	SWS_FT_FI11
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17081983, ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163

Chain	Residue	Details
4	SER120

---

site_id	SWS_FT_FI12
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163

Chain	Residue	Details
4	SER131

---

site_id	SWS_FT_FI13
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:20068231

Chain	Residue	Details
4	SER142
4	SER145

---

site_id	SWS_FT_FI14
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
4	LYS220
4	LYS450

---

site_id	SWS_FT_FI15
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P49717

Chain	Residue	Details
4	LYS858

---

site_id	SWS_FT_FI16
Number of Residues	3
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
4	LYS439
3	SER779
4	LYS798

---

site_id	SWS_FT_FI17
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:16899510, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569

Chain	Residue	Details
2	SER139

---

site_id	SWS_FT_FI18
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
2	LYS216

---

site_id	SWS_FT_FI19
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:21406692

Chain	Residue	Details
2	SER381

---

site_id	SWS_FT_FI20
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
2	LYS178

---