6XMJ
Human 20S proteasome bound to an engineered 11S (PA26) activator
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Proteasome subunit alpha type-6 | polymer | 244 | 27186.2 | 1 | UniProt (P60900) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (Human) | 27 kDa prosomal protein,p27K,Macropain iota chain,Multicatalytic endopeptidase complex iota chain,Proteasome iota chain |
| 2 | B (B) | Proteasome subunit alpha type-2 | polymer | 233 | 25796.3 | 1 | UniProt (P25787) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (Human) | Macropain subunit C3,Multicatalytic endopeptidase complex subunit C3,Proteasome component C3 |
| 3 | C (C) | Proteasome subunit alpha type-4 | polymer | 250 | 28118.2 | 1 | UniProt (P25789) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (Human) | Macropain subunit C9,Multicatalytic endopeptidase complex subunit C9,Proteasome component C9,Proteasome subunit L |
| 4 | D (D) | Proteasome subunit alpha type-7 | polymer | 243 | 27382.2 | 1 | UniProt (O14818) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (Human) | Proteasome subunit RC6-1,Proteasome subunit XAPC7 |
| 5 | E (E) | Proteasome subunit alpha type-5 | polymer | 234 | 25570.0 | 1 | UniProt (P28066) Pfam (PF00227) Pfam (PF10584) | Homo sapiens (Human) | Macropain zeta chain,Multicatalytic endopeptidase complex zeta chain,Proteasome zeta chain |
| 6 | F (F) | Proteasome subunit alpha type-1 | polymer | 238 | 26728.4 | 1 | UniProt (P25786) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (Human) | 30 kDa prosomal protein,PROS-30,Macropain subunit C2,Multicatalytic endopeptidase complex subunit C2,Proteasome component C2,Proteasome nu chain |
| 7 | G (G) | Proteasome subunit alpha type-3 | polymer | 245 | 27287.1 | 1 | UniProt (P25788) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (Human) | Macropain subunit C8,Multicatalytic endopeptidase complex subunit C8,Proteasome component C8 |
| 8 | H (H) | Proteasome subunit beta type-6 | polymer | 202 | 21656.5 | 1 | UniProt (P28072) Pfam (PF00227) | Homo sapiens (Human) | Macropain delta chain,Multicatalytic endopeptidase complex delta chain,Proteasome delta chain,Proteasome subunit Y |
| 9 | I (I) | Proteasome subunit beta type-7 | polymer | 220 | 23745.3 | 1 | UniProt (Q99436) Pfam (PF00227) Pfam (PF12465) | Homo sapiens (Human) | Macropain chain Z,Multicatalytic endopeptidase complex chain Z,Proteasome subunit Z |
| 10 | J (J) | Proteasome subunit beta type-3 | polymer | 204 | 22841.7 | 1 | UniProt (P49720) Pfam (PF00227) | Homo sapiens (Human) | Proteasome chain 13,Proteasome component C10-II,Proteasome theta chain |
| 11 | K (K) | Proteasome subunit beta type-2 | polymer | 199 | 22720.1 | 1 | UniProt (P49721) Pfam (PF00227) | Homo sapiens (Human) | Macropain subunit C7-I,Multicatalytic endopeptidase complex subunit C7-I,Proteasome component C7-I |
| 12 | L (L) | Proteasome subunit beta type-5 | polymer | 201 | 22199.1 | 1 | UniProt (P28074) Pfam (PF00227) | Homo sapiens (Human) | Macropain epsilon chain,Multicatalytic endopeptidase complex epsilon chain,Proteasome chain 6,Proteasome epsilon chain,Proteasome subunit MB1,Proteasome subunit X |
| 13 | M (M) | Proteasome subunit beta type-1 | polymer | 213 | 23579.0 | 1 | UniProt (P20618) Pfam (PF00227) | Homo sapiens (Human) | Macropain subunit C5,Multicatalytic endopeptidase complex subunit C5,Proteasome component C5,Proteasome gamma chain |
| 14 | N (N) | Proteasome subunit beta type-4 | polymer | 217 | 24138.5 | 1 | UniProt (P28070) Pfam (PF00227) | Homo sapiens (Human) | 26 kDa prosomal protein,PROS-26,Macropain beta chain,Multicatalytic endopeptidase complex beta chain,Proteasome beta chain,Proteasome chain 3,HsN3 |
| 15 | O, P, Q, R, S... (O, P, Q, R, S...) | Proteasome activator protein PA26 | polymer | 228 | 24959.4 | 7 | UniProt (Q9U8G2) Pfam (PF02252) | Trypanosoma brucei |
Sequence modifications
O, P, Q, R, S, T, U: 4 - 226 (UniProt: Q9U8G2)
| PDB | External Database | Details |
|---|---|---|
| Val 49 | Thr 49 | engineered mutation |
| Ala 102 | Glu 102 | engineered mutation |
| Asn 226 | Ser 226 | engineered mutation |
| Leu 227 | - | expression tag |
| Ser 228 | - | expression tag |
| Tyr 229 | - | expression tag |
| Tyr 230 | - | expression tag |
| Thr 231 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 21 |
| Total formula weight | 523664.1 | |
| All* | Total formula weight | 523664.1 |
