6XHA
Crystal Structure of KRAS-G12V (GMPPNP-bound) in complex with RAS-binding domain (RBD) and cysteine-rich domain (CRD) of RAF1/CRAF
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Isoform 2B of GTPase KRas | polymer | 170 | 19354.8 | 1 | UniProt (P01116-2) Pfam (PF00071) UniProt (by SIFTS) (P01116) | Homo sapiens (Human) | K-Ras 2,Ki-Ras,c-K-ras,c-Ki-ras |
| 2 | B (B) | RAF proto-oncogene serine/threonine-protein kinase | polymer | 137 | 15888.4 | 1 | UniProt (P04049) Pfam (PF02196) Pfam (PF00130) | Homo sapiens (Human) | Proto-oncogene c-RAF,cRaf,Raf-1 |
| 3 | C (A) | PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER | non-polymer | 522.2 | 1 | Chemie (GNP) | |||
| 4 | D, G, H, I (A, B) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 5 | E (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 6 | F, L (A, B) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 7 | J, K (B) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 8 | M, N (A, B) | water | water | 18.0 | 12 | Chemie (HOH) |
Sequence modifications
A: 1 - 169 (UniProt: P01116-2)
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Val 12 | Gly 12 | engineered mutation |
| Ser 118 | Cys 118 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 35243.3 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1116.6 | |
| All* | Total formula weight | 36359.9 |
