6X91

Crystal structure of MBP-fused human APOBEC1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Maltodextrin-binding protein, C->U-editing enzyme APOBEC-1 chimera	polymer	593	67073.3	8	UniProt (A0A4Z0THX4) UniProt (P41238) Pfam (PF01547) Pfam (PF05240) UniProt (by SIFTS) (P0AEX9) In PDB	Escherichia coli	Apolipoprotein B mRNA-editing enzyme 1,HEPR,mRNA(cytosine(6666)) deaminase 1
2	I, J, K, L, M...	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	14	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
3	C, D, E, F, G...	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
4	C, D, E, F, G...	CACODYLATE ION	non-polymer		137.0	8	Chemie (CAC)

Sequence modifications

A, B, C, D, E, F, G, H: 2 - 367 (UniProt: A0A4Z0THX4)

PDB	External Database	Details
Met 1	-	initiating methionine
Val 313	Ala 338	engineered mutation
Asn 368	-	linker
Ser 369	-	linker
Ser 370	-	linker
Ser 371	-	linker

A, B, C, D, E, F, G, H: 1015 - 1236 (UniProt: P41238)

PDB	External Database	Details
Ala 1046	Met 46	engineered mutation
Ser 1048	Arg 48	engineered mutation
Ala 1063	Glu 63	engineered mutation
Thr 1080	Met 80	engineered mutation
Ala 1121	Trp 121	conflict
Asp 1146	Ala 146	engineered mutation
Ala 1173	Leu 173	engineered mutation
Ala 1199	Trp 199	engineered mutation
Ala 1205	Phe 205	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	536586.5
Branched	Number of molecules	14
	Total formula weight	4792.2
Non-Polymers*	Number of molecules	16
	Total formula weight	1619.2
All*	Total formula weight	542997.8

*Water molecules are not included.