6X91

Crystal structure of MBP-fused human APOBEC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.28345
Spacegroup name	P 21 21 2
Unit cell lengths	177.715, 179.209, 210.505
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.610 - 3.510
R-factor	0.2784
Rwork	0.276
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 1ANF & 2NYT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.560
High resolution limit [Å]	3.500	3.500
Rmerge		1.150
Number of reflections	83654	4100
<I/σ(I)>	5.22
Completeness [%]	99.2	98.1
Redundancy	4.9	3.7
CC(1/2)	0.883	0.213

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	0.2 M potassium chloride, 0.2 M magnesium acetate tetrahydrate, 0.05 M sodium cacodylate, pH 6.0, 8.5% PEG8000