6WWZ

Cryo-EM structure of the human chemokine receptor CCR6 in complex with CCL20 and a Go protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P54311) Pfam (PF00400)	Rattus norvegicus (Norway rat)	Transducin beta chain 1
2	B (C)	C-C motif chemokine 20	polymer	70	8043.6	1	UniProt (P78556) Pfam (PF00048)	Homo sapiens (Human)	Beta-chemokine exodus-1,CC chemokine LARC,Liver and activation-regulated chemokine,Macrophage inflammatory protein 3 alpha,MIP-3-alpha,Small-inducible cytokine A20
3	C (Y)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7563.8	1	Pfam (PF00631) UniProt (by SIFTS) (P59768)	Rattus norvegicus (Norway rat)
4	D (A)	Guanine nucleotide-binding protein G(o) subunit alpha,Guanine nucleotide-binding protein G(o) subunit alpha	polymer	250	28195.9	1	UniProt (P09471) Pfam (PF00503)	Homo sapiens (Human)
5	E (S)	scFv16	polymer	287	30895.5	1		Mus musculus (Mouse)
6	F (R)	C-C chemokine receptor type 6,C-C chemokine receptor type 6	polymer	529	60064.6	1	UniProt (P51684) Pfam (PF07361) Pfam (PF00001) UniProt (by SIFTS) (P0ABE7)	Homo sapiens (Human)	CCR-6,Chemokine receptor-like 3,CKR-L3,DRY6,G-protein coupled receptor 29,GPR-CY4,GPRCY4,LARC receptor

Sequence modifications

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

A: 4 - 173 (UniProt: P09471)

PDB	External Database	Details
Met -11	-	initiating methionine
Gly -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Glu -3	-	expression tag
Asn -2	-	expression tag
Leu -1	-	expression tag
Tyr 0	-	expression tag
Phe 1	-	expression tag
Gln 2	-	expression tag
Gly 3	-	expression tag
Asp 42	Gly 42	conflict
Asn 43	Glu 43	conflict

A: 182 - 354 (UniProt: P09471)

PDB	External Database	Details
Gly 174	-	linker
Gly 175	-	linker
Ser 176	-	linker
Gly 177	-	linker
Gly 178	-	linker
Ser 179	-	linker
Gly 180	-	linker
Gly 181	-	linker
Asp 227	Ala 227	conflict
Asp 230	Gly 230	conflict
Asp 250	Leu 250	conflict
Ala 332	Ile 332	conflict
Ile 335	Val 335	conflict

R: -144 - 1 (PDB: 6WWZ)
R: 2 - 374 (UniProt: P51684)

PDB	External Database	Details
His 375	-	expression tag
His 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag
His 379	-	expression tag
His 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	173507.7
All*	Total formula weight	173507.7

*Water molecules are not included.