6WPF

Structure of HIV-1 Reverse Transcriptase (RT) in complex with dsDNA and d4T

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	P	DNA Primer 21-mer	polymer	21	6476.2	1		synthetic construct
2	T	DNA template 27-mer	polymer	27	8392.4	1		synthetic construct
3	A	Reverse transcriptase/ribonuclease H	polymer	561	64653.1	1	UniProt (P04585) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075) In PDB	Human immunodeficiency virus type 1 group M subtype B (isolate HXB2) (HIV-1)	Pr160Gag-Pol
4	B	p51 RT	polymer	452	52748.4	1	UniProt (P04585) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) In PDB	Human immunodeficiency virus type 1 group M subtype B (isolate HXB2) (HIV-1)
5	P	[(5R)-5-(5-METHYL-2,4-DIOXO-3,4-DIHYDROPYRIMIDIN-1(2H)-YL)-2,5-DIHYDROFURAN-2-YL]METHYL DIHYDROGEN PHOSPHATE	non-polymer		304.2	1	Chemie (D4M)
6	P, T, A	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
7	P, A	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
8	A	2',3'-DEHYDRO-2',3'-DEOXY-THYMIDINE 5'-TRIPHOSPHATE	non-polymer		464.2	1	Chemie (D4T)
9	B	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
10		water	water		18.0	139	Chemie (HOH)

Sequence modifications

A: 1 - 560 (UniProt: P04585)

PDB	External Database	Details
Met 0	-	expression tag
Cys 258	Gln 845	engineered mutation
Ser 280	Cys 867	engineered mutation

B: 1 - 440 (UniProt: P04585)

PDB	External Database	Details
Met -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Ser 280	Cys 867	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	132270.1
Non-Polymers*	Number of molecules	10
	Total formula weight	1317.6
All*	Total formula weight	133587.8

*Water molecules are not included.