6WKR

PRC2-AEBP2-JARID2 bound to H2AK119ub1 nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	T, F	Ubiquitin	polymer	76	8622.9	2	UniProt (P0CG48) Pfam (PF00240) In PDB	Homo sapiens (Human)
2	A	Polycomb protein SUZ12	polymer	739	83181.9	1	UniProt (Q15022) Pfam (PF09733) In PDB	Homo sapiens (Human)	Chromatin precipitated E2F target 9 protein,ChET 9 protein,Joined to JAZF1 protein,Suppressor of zeste 12 protein homolog
3	L	Polycomb protein EED	polymer	441	50267.7	1	UniProt (O75530) Pfam (PF00400) In PDB	Homo sapiens (Human)	hEED,Embryonic ectoderm development protein,WD protein associating with integrin cytoplasmic tails 1,WAIT-1
4	N	Histone-binding protein RBBP4	polymer	425	47709.5	1	UniProt (Q09028) Pfam (PF12265) Pfam (PF00400) In PDB	Homo sapiens (Human)	Chromatin assembly factor 1 subunit C,CAF-1 subunit C,Chromatin assembly factor I p48 subunit,CAF-I p48,Nucleosome-remodeling factor subunit RBAP48,Retinoblastoma-binding protein 4,RBBP-4,Retinoblastoma-binding protein p48
5	C	Histone-lysine N-methyltransferase EZH2	polymer	746	85492.3	1	UniProt (Q15910) Pfam (PF11616) Pfam (PF18118) Pfam (PF21358) Pfam (PF18264) Pfam (PF00856) In PDB	Homo sapiens (Human)	ENX-1,Enhancer of zeste homolog 2,Lysine N-methyltransferase 6
6	B, E	Protein Jumonji	polymer	450	49306.1	2	UniProt (Q92833) In PDB	Homo sapiens (Human)	Jumonji/ARID domain-containing protein 2
7	P	Zinc finger protein AEBP2	polymer	295	33012.7	1	UniProt (Q6ZN18) In PDB	Homo sapiens (Human)	Adipocyte enhancer-binding protein 2,AE-binding protein 2
8	H	DNA (314-MER)	polymer	320	98186.1	1		Homo sapiens
9	I, O	Histone H3.2	polymer	136	15421.1	2	UniProt (P84233) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)	Histone H3
10	J, Q	Histone H4	polymer	103	11394.4	2	UniProt (P62799) Pfam (PF15511) In PDB	Xenopus laevis (African clawed frog)
11	K, R	Histone H2A type 1	polymer	130	14067.4	2	UniProt (P06897) Pfam (PF00125) Pfam (PF16211) In PDB	Xenopus laevis (African clawed frog)
12	M, S	Histone H2B 1.1	polymer	126	13979.3	2	UniProt (P02281) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)	H2B1.1
13	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
14	C	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)
15	P	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

T, F: 1 - 76 (UniProt: P0CG48)

PDB	External Database	Details
Cys 76	Gly 76	engineered mutation

K, R: 0 - 129 (UniProt: P06897)

PDB	External Database	Details
Arg 99	Gly 100	conflict
Cys 119	Lys 120	conflict

M, S: -3 - 122 (UniProt: P02281)

PDB	External Database	Details
Thr 29	Ser 33	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	18
	Total formula weight	623432.6
Non-Polymers*	Number of molecules	3
	Total formula weight	474.1
All*	Total formula weight	623906.7

*Water molecules are not included.