6W9A

RNF12 RING domain in complex with Ube2e2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Ubiquitin-conjugating enzyme E2 E2	polymer	177	19667.5	2	UniProt (Q96LR5) Pfam (PF00179)	Homo sapiens (Human)	E2 ubiquitin-conjugating enzyme E2,UbcH8,Ubiquitin carrier protein E2,Ubiquitin-protein ligase E2
2	B, D (B, D)	E3 ubiquitin-protein ligase RLIM	polymer	98	11043.4	2	UniProt (Q9NVW2) Pfam (PF13639)	Homo sapiens (Human)	LIM domain-interacting RING finger protein,RING finger LIM domain-binding protein,R-LIM,RING finger protein 12,RING-type E3 ubiquitin transferase RLIM,Renal carcinoma antigen NY-REN-43, RNF12
3	E, H (A, C)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	F, G, I, J (B, D)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5	K, L, M, N (A, B, C, D)	water	water		18.0	28	Chemie (HOH)

Sequence modifications

A, C: 30 - 201 (UniProt: Q96LR5)

PDB	External Database	Details
Gly 25	-	expression tag
Pro 26	-	expression tag
Leu 27	-	expression tag
Gly 28	-	expression tag
Ser 29	-	expression tag

B, D: 530 - 623 (UniProt: Q9NVW2)

PDB	External Database	Details
Gly 526	-	expression tag
Pro 527	-	expression tag
Leu 528	-	expression tag
Gly 529	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	61421.8
Non-Polymers*	Number of molecules	6
	Total formula weight	445.8
All*	Total formula weight	61867.6

*Water molecules are not included.