6W9A
RNF12 RING domain in complex with Ube2e2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-25 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 31 |
Unit cell lengths | 81.355, 81.355, 98.247 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.296 - 2.300 |
R-factor | 0.182 |
Rwork | 0.180 |
R-free | 0.21860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y6l |
RMSD bond length | 0.008 |
RMSD bond angle | 0.959 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.29) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13-2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.680 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.081 | 1.129 |
Rmeas | 0.089 | 1.239 |
Rpim | 0.037 | 0.510 |
Total number of observations | 188008 | |
Number of reflections | 32329 | 3171 |
<I/σ(I)> | 15.9 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 5.8 | 5.8 |
CC(1/2) | 0.999 | 0.629 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 20% PEG 3350, 100 mM Na/K phosphate, 100 mM bis-Tris propane |