6W6W

Cryo-EM structure of CST bound to telomeric single-stranded DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	CST complex subunit CTC1	polymer	1233	136746.0	2	UniProt (Q2NKJ3) Pfam (PF15489)	Homo sapiens (Human)	Conserved telomere maintenance component 1,HBV DNAPTP1-transactivated protein B
2	B (E)	DNA (5'-D(P*TP*AP*GP*G)-3')	polymer	4	1230.9	1		Homo sapiens
3	D (C)	CST complex subunit STN1	polymer	374	43001.8	1	UniProt (Q9H668) Pfam (PF01336) Pfam (PF09170)	Homo sapiens (Human)	Oligonucleotide/oligosaccharide-binding fold-containing protein 1,Suppressor of cdc thirteen homolog
4	E (D)	CST complex subunit TEN1	polymer	152	17285.6	1	UniProt (Q86WV5) Pfam (PF15490)	Homo sapiens (Human)	Protein telomeric pathways with STN1 homolog,Telomere length regulation protein TEN1 homolog
5	F (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A, B: 2 - 1217 (UniProt: Q2NKJ3)

PDB	External Database	Details
Met -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Lys -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Asp -9	-	expression tag
Asp -8	-	expression tag
Lys -7	-	expression tag
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Lys 1	-	expression tag

C: 2 - 368 (UniProt: Q9H668)

PDB	External Database	Details
Met -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag

D: 3 - 123 (UniProt: Q86WV5)

PDB	External Database	Details
Met -28	-	expression tag
Ser -27	-	expression tag
Tyr -26	-	expression tag
Tyr -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
Asp -18	-	expression tag
Tyr -17	-	expression tag
Asp -16	-	expression tag
Ile -15	-	expression tag
Pro -14	-	expression tag
Thr -13	-	expression tag
Thr -12	-	expression tag
Glu -11	-	expression tag
Asn -10	-	expression tag
Leu -9	-	expression tag
Tyr -8	-	expression tag
Phe -7	-	expression tag
Gln -6	-	expression tag
Gly -5	-	expression tag
Ala -4	-	expression tag
Met -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Ile 1	-	expression tag
Gln 2	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	335010.3
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	335075.7

*Water molecules are not included.