6W51

Structure of the antibody fragment H2 in complex with HLA-A*02:01/p53R175H

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G, J (A, D, G, J)	MHC class I antigen	polymer	296	34183.7	4	UniProt (U5YKE0) Pfam (PF00129) Pfam (PF07654) UniProt (by SIFTS) (A0A140T913)	Homo sapiens (Human)
2	B, E, H, K (B, E, H, K)	Beta-2-microglobulin	polymer	119	13732.5	4	UniProt (P61769) Pfam (PF07654)	Homo sapiens (Human)
3	C, F, I, L (C, F, I, L)	Cellular tumor antigen p53 peptide	polymer	9	1114.3	4	UniProt (P04637)	Homo sapiens	Antigen NY-CO-13,Phosphoprotein p53,Tumor suppressor p53
4	M, O, Q, S (M, O, Q, S)	Immunoglobulin heavy chain H2	polymer	223	23780.5	4		Homo sapiens (Human)
5	N, P, R, T (N, P, R, T)	Immunoglobulin light chain H2	polymer	215	23698.3	4		Homo sapiens (Human)
6	U (S)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
7	V (N)	water	water		18.0	1	Chemie (HOH)

Sequence modifications

A, D, G, J: 1 - 276 (UniProt: U5YKE0)

PDB	External Database	Details
Met 0	-	initiating methionine
Gly 277	-	expression tag
Ser 278	-	expression tag
Gly 279	-	expression tag
Ser 280	-	expression tag
Gly 281	-	expression tag
Leu 282	-	expression tag
Asn 283	-	expression tag
Asp 284	-	expression tag
Ile 285	-	expression tag
Phe 286	-	expression tag
Glu 287	-	expression tag
Ala 288	-	expression tag
Gln 289	-	expression tag
Lys 290	-	expression tag
Ile 291	-	expression tag
Glu 292	-	expression tag
Trp 293	-	expression tag
His 294	-	expression tag
Glu 295	-	expression tag

C, F, I, L: 1 - 9 (UniProt: P04637)

PDB	External Database	Details
His 8	Arg 175	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	20
	Total formula weight	386037.7
Non-Polymers*	Number of molecules	1
	Total formula weight	95.0
All*	Total formula weight	386132.7

*Water molecules are not included.