6VZI
Crystal Structure of HIV-1 CAP256 RnS-3mut-2G-SOSIP.664 Prefusion Env Trimer in Complex with Human Antibodies 3H109L and 35O22 at 3.5 Angstrom
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Envelope glycoprotein gp41 | polymer | 154 | 17324.7 | 1 | UniProt (W6ICH7) | Human immunodeficiency virus 1 | |
| 2 | B (D) | 35O22 scFv heavy chain | polymer | 134 | 14663.3 | 1 | Homo sapiens (Human) | ||
| 3 | C (E) | 35O22 scFv light chain | polymer | 114 | 12358.6 | 1 | Homo sapiens (Human) | ||
| 4 | D (G) | Envelope glycoprotein gp160 | polymer | 471 | 53421.6 | 1 | UniProt (A0A0N9FF17) | Human immunodeficiency virus 1 | Env polyprotein |
| 5 | E (H) | 3H109L Fab heavy chain | polymer | 244 | 26255.5 | 1 | Homo sapiens (Human) | ||
| 6 | F (L) | 3H109L Fab light chain | polymer | 217 | 23416.1 | 1 | Homo sapiens (Human) | ||
| 7 | G (A) | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 1 | In PDB GlyTouCan (G09724ZC) | |||
| 8 | H, I (C, F) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 2 | In PDB GlyTouCan (G15407YE) | |||
| 9 | J, L, M (I, K, M) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 10 | K (J) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1721.5 | 1 | In PDB GlyTouCan (G40702WU) | |||
| 11 | N, O, P, Q, R... (G) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 8 | Chemie (NAG) |
Sequence modifications
B: 511 - 664 (UniProt: W6ICH7)
G: 33 - 507 (UniProt: A0A0N9FF17)
| PDB | External Database | Details |
|---|---|---|
| Asn 535 | Ile 528 | engineered mutation |
| Pro 559 | Ile 552 | engineered mutation |
| Gly 569 | Thr 562 | engineered mutation |
| Phe 573 | Ile 566 | engineered mutation |
| Glu 588 | Lys 581 | engineered mutation |
| Val 589 | Asp 582 | engineered mutation |
| Cys 605 | Thr 598 | engineered mutation |
| Pro 609 | Tyr 602 | engineered mutation |
| Gly 636 | Asp 629 | engineered mutation |
| Phe 651 | Lys 644 | engineered mutation |
| Ile 655 | Ser 648 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ile 204 | Ala 196 | engineered mutation |
| Met 302 | Asn 294 | engineered mutation |
| Leu 320 | Thr 310 | engineered mutation |
| Pro 329 | Ala 320 | engineered mutation |
| Pro 437 | Ser 423 | engineered mutation |
| Asn 442 | Glu 428 | engineered mutation |
| Cys 501 | Ala 487 | engineered mutation |
| Arg 508 | - | expression tag |
| Arg 509 | - | expression tag |
| Arg 510 | - | expression tag |
| Arg 511 | - | expression tag |
| Arg 512 | - | expression tag |
| Arg 513 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 147439.9 | |
| Branched | Number of molecules | 7 |
| Total formula weight | 5240.8 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1769.7 | |
| All* | Total formula weight | 154450.4 |
