6VZI

Crystal Structure of HIV-1 CAP256 RnS-3mut-2G-SOSIP.664 Prefusion Env Trimer in Complex with Human Antibodies 3H109L and 35O22 at 3.5 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-13
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	P 63
Unit cell lengths	133.920, 133.920, 315.110
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.836 - 2.716
R-factor	0.2318
Rwork	0.229
R-free	0.27550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mtj
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.128	0.066	2.067
Rmeas	0.138	0.070	2.368
Rpim	0.049	0.024	1.122
Total number of observations	383837
Number of reflections	53716	8493	1231
<I/σ(I)>	5.5
Completeness [%]	63.3	98.9	14.6
Redundancy	7.1	8.5	3.2
CC(1/2)		0.990	0.113

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		298	60 mM sodium acetate pH 4.6, 120 mM ammonium sulfate and 6.3% PEG 4,000