6VRD

Crystal structure of RNase H/RNA/PS-ASO complex at an atomic level

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ribonuclease H1	polymer	294	33218.5	1	UniProt (O60930) Pfam (PF01693) Pfam (PF00075)	Homo sapiens (Human)	RNase H1,Ribonuclease H type II
2	B (B)	RNA (5'-R(*UP*GP*GP*CP*GP*AP*GP*UP*GP*GP*GP*UP*GP*AP*GP*UP*GP*AP*GP*G)-3')	polymer	20	6615.0	1		Homo sapiens
3	C (C)	RNA (5'-R(*(OMC)P*(N7X)P*(T39)P*(C5L)P*(A2M))-D(P*(SC)P*(PST)P*(SC)P*(AS)P*(SC)P*(SC)P*(SC)P*(AS)P*(SC)P*(PST))-R(P*(6OO)P*(RFJ)P*(6OO)P*(6OO)P*(6NW))-3')	polymer	20	6637.4	1		synthetic construct
4	D, E, H (A, C)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
5	F (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6	G (C)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
7	I, J, K (A, B, C)	water	water		18.0	350	Chemie (HOH)

Sequence modifications

A: 1 - 286 (UniProt: O60930)

PDB	External Database	Details
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Asn 210	Asp 210	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	46470.8
Non-Polymers*	Number of molecules	5
	Total formula weight	486.4
All*	Total formula weight	46957.2

*Water molecules are not included.