6VRD

Crystal structure of RNase H/RNA/PS-ASO complex at an atomic level

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97856
Spacegroup name	P 21 21 21
Unit cell lengths	46.760, 53.030, 106.477
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.133 - 1.299
R-factor	0.1914
Rwork	0.190
R-free	0.22860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qk9
Data reduction software	XDS (vJan10,2014)
Data scaling software	Aimless (v0.7.4)
Phasing software	PHASER (v2.8.3)
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.133	1.346
High resolution limit [Å]	1.299	1.299
Number of reflections	65937	6385
<I/σ(I)>	12.67
Completeness [%]	99.8
Redundancy	2
CC(1/2)	1.000	0.397

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.8 M Ammonium sulphate, 0.1 M citrate pH 4.0