6VGG

Crystal structure of the DNA binding domains of human transcription factor ERG, human Runx2 bound to core binding factor beta (Cbfb), and mithramycin, in complex with 16mer DNA CAGAGGATGTGGCTTC

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Transcriptional regulator ERG	polymer	128	14927.8	1	UniProt (P11308) Pfam (PF00178) In PDB	Homo sapiens (Human)	Transforming protein ERG
2	B	DNA (5'-D(P*CP*AP*GP*AP*GP*GP*AP*TP*GP*TP*GP*GP*CP*TP*TP*C)-3')	polymer	16	4954.2	1		Homo sapiens
3	C	DNA (5'-D(P*GP*AP*AP*GP*CP*CP*AP*CP*AP*TP*CP*CP*TP*CP*TP*G)-3')	polymer	16	4843.2	1		Homo sapiens
4	D	Runt-related transcription factor 2	polymer	177	19542.1	1	UniProt (Q13950) Pfam (PF00853) In PDB	Homo sapiens (Human)	Acute myeloid leukemia 3 protein,Core-binding factor subunit alpha-1,CBF-alpha-1,Oncogene AML-3,Osteoblast-specific transcription factor 2,OSF-2,Polyomavirus enhancer-binding protein 2 alpha A subunit,PEBP2-alpha A,SL3-3 enhancer factor 1 alpha A subunit,SL3/AKV core-binding factor alpha A subunit
5	G	Core-binding factor subunit beta	polymer	156	18263.4	1	UniProt (Q13951) Pfam (PF02312) In PDB	Homo sapiens (Human)	CBF-beta,Polyomavirus enhancer-binding protein 2 beta subunit,PEBP2-beta,SL3-3 enhancer factor 1 subunit beta,SL3/AKV core-binding factor beta subunit
6	B, C	mithramycin	non-polymer		1085.1	2	Chemie (QWP)
7	B	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
8		water	water		18.0	2	Chemie (HOH)

Sequence modifications

A: 306 - 419 (UniProt: P11308)

PDB	External Database	Details
Gly 302	-	expression tag
Pro 303	-	expression tag
His 304	-	expression tag
Met 305	-	expression tag
Ser 420	-	expression tag
Tyr 421	-	expression tag
His 422	-	expression tag
Ala 423	-	expression tag
His 424	-	expression tag
Pro 425	-	expression tag
Gln 426	-	expression tag
Lys 427	-	expression tag
Met 428	-	expression tag
Asn 429	-	expression tag

G: 1 - 142 (UniProt: Q13951)

PDB	External Database	Details
Met -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	62530.7
Non-Polymers*	Number of molecules	3
	Total formula weight	2194.6
All*	Total formula weight	64725.3

*Water molecules are not included.