6VC7

Structure of the F349A mutant of the periplasmic domain of YejM from Salmonella typhimurium

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Periplasmic domain of the cardiolipin transporter protein YejM/PbgA	polymer	368	41097.7	6	UniProt (A0A5A8TP41) Pfam (PF00884) UniProt (by SIFTS) (P40709) In PDB	Salmonella typhimurium
2	C, A, B	HEXAETHYLENE GLYCOL	non-polymer		282.3	3	Chemie (P6G)
3	A, B	TRIETHYLENE GLYCOL	non-polymer		150.2	2	Chemie (PGE)
4	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5	F, C, A, D, E...	ETHANOLAMINE	non-polymer		61.1	6	Chemie (ETA)
6	F, C, A, D, B...	1,2-ETHANEDIOL	non-polymer		62.1	21	Chemie (EDO)
7	B, F, D, A, E	TETRAETHYLENE GLYCOL	non-polymer		194.2	7	Chemie (PG4)
8	B, F, C, D, A...	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
9	B, D, A	POTASSIUM ION	non-polymer		39.1	3	Chemie (K)
10	C, D, E	PENTAETHYLENE GLYCOL	non-polymer		238.3	3	Chemie (1PE)
11		water	water		18.0	1010	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 241 - 586 (UniProt: A0A5A8TP41)

PDB	External Database	Details
Met 219	-	expression tag
Ser 220	-	expression tag
Gly 221	-	expression tag
His 222	-	expression tag
His 223	-	expression tag
His 224	-	expression tag
His 225	-	expression tag
His 226	-	expression tag
His 227	-	expression tag
Ser 228	-	expression tag
Ser 229	-	expression tag
Gly 230	-	expression tag
Leu 231	-	expression tag
Val 232	-	expression tag
Pro 233	-	expression tag
Arg 234	-	expression tag
Gly 235	-	expression tag
Ser 236	-	expression tag
His 237	-	expression tag
Met 238	-	expression tag
Ala 239	-	expression tag
Ser 240	-	expression tag
Ala 349	Phe 349	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	246586.2
Non-Polymers*	Number of molecules	52
	Total formula weight	5260.9
All*	Total formula weight	251847.2

*Water molecules are not included.