6VC7
Structure of the F349A mutant of the periplasmic domain of YejM from Salmonella typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 80 |
Detector technology | CMOS |
Collection date | 2018-03-14 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.001 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 121.612, 124.899, 183.296 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.560 - 2.050 |
R-factor | 0.1866 |
Rwork | 0.184 |
R-free | 0.22680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6vat |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.670 | 2.123 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 150136 | 8464 |
<I/σ(I)> | 13.8 | |
Completeness [%] | 85.9 | 48.97 |
Redundancy | 6.4 | |
CC(1/2) | 0.997 | 0.184 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 291 | 0.1 M HEPES pH 6.8, 5% PEG3350 |