6V2J

Crystal structure of ClC-ec1 triple mutant (E113Q, E148Q, E203Q)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	H(+)/Cl(-) exchange transporter ClcA	polymer	489	52112.4	1	UniProt (P37019) Pfam (PF00654)	Escherichia coli (strain K12)	ClC-ec1
2	B, C, D (A)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
3	E (A)	water	water		18.0	55	Chemie (HOH)

Sequence modifications

A: 1 - 468 (UniProt: P37019)

PDB	External Database	Details
Gln 113	Glu 113	engineered mutation
Gln 148	Glu 148	engineered mutation
Gln 203	Glu 203	engineered mutation
Lys 469	-	expression tag
Gly 470	-	expression tag
Ser 471	-	expression tag
Gly 472	-	expression tag
Thr 473	-	expression tag
Leu 474	-	expression tag
Val 475	-	expression tag
Pro 476	-	expression tag
Arg 477	-	expression tag
Gly 478	-	expression tag
Ser 479	-	expression tag
Gly 480	-	expression tag
Gly 481	-	expression tag
Leu 482	-	expression tag
Glu 483	-	expression tag
His 484	-	expression tag
His 485	-	expression tag
His 486	-	expression tag
His 487	-	expression tag
His 488	-	expression tag
His 489	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	52112.4
Non-Polymers*	Number of molecules	3
	Total formula weight	106.4
All*	Total formula weight	52218.8

*Water molecules are not included.