6US5
DNA polymerase I Large Fragment from Bacillus stearothermophilus with DNA template, 3'-amino primer, dGpNHpp analog, and Mn2+
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D | DNA polymerase I | polymer | 579 | 66073.8 | 2 | UniProt (D9N168) Pfam (PF22619) Pfam (PF00476) In PDB | Geobacillus stearothermophilus | |
| 2 | B, E | DNA (5'-D(*GP*CP*GP*AP*TP*CP*AP*GP*(C42))-3') | polymer | 9 | 2739.8 | 2 | synthetic construct | ||
| 3 | C, F | DNA (5'-D(*CP*AP*CP*GP*CP*TP*GP*AP*TP*CP*GP*CP*A)-3') | polymer | 13 | 3936.6 | 2 | synthetic construct | ||
| 4 | A, D | 2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]guanosine | non-polymer | 506.2 | 2 | Chemie (XG4) | |||
| 5 | A, D | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) | |||
| 6 | A, C, D | SULFATE ION | non-polymer | 96.1 | 7 | Chemie (SO4) | |||
| 7 | water | water | 18.0 | 410 | Chemie (HOH) |
Sequence modifications
A, D: 298 - 876 (UniProt: D9N168)
| PDB | External Database | Details |
|---|---|---|
| Tyr 710 | Phe 413 | engineered mutation |
| Val 713 | Pro 416 | variant |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 145500.3 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 1794.7 | |
| All* | Total formula weight | 147295.0 |
