6URO
Cryo-EM structure of human CPSF160-WDR33-CPSF30-PAS RNA-CstF77 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Cleavage and polyadenylation specificity factor subunit 1 | polymer | 1443 | 161074.2 | 1 | UniProt (Q10570) Pfam (PF10433) Pfam (PF03178) | Homo sapiens (Human) | Cleavage and polyadenylation specificity factor 160 kDa subunit, CPSF 160 kDa subunit |
| 2 | B (B) | pre-mRNA 3' end processing protein WDR33 | polymer | 587 | 67546.8 | 1 | UniProt (Q9C0J8) Pfam (PF00400) | Homo sapiens (Human) | WD repeat-containing protein 33, WD repeat-containing protein WDC146 |
| 3 | C (C) | Cleavage and polyadenylation specificity factor subunit 4 | polymer | 250 | 28417.9 | 1 | UniProt (O95639) Pfam (PF00642) Pfam (PF00098) Pfam (PF15663) | Homo sapiens (Human) | Cleavage and polyadenylation specificity factor 30 kDa subunit,CPSF 30 kDa subunit,NS1 effector domain-binding protein 1,Neb-1,No arches homolog |
| 4 | D (D) | PAS RNA | polymer | 47 | 14823.7 | 1 | Macaca mulatta polyomavirus 1 | ||
| 5 | E, F (E, F) | Cleavage stimulation factor subunit 3 | polymer | 717 | 83039.6 | 2 | UniProt (Q12996) Pfam (PF05843) | Homo sapiens (Human) | CF-1 77 kDa subunit,Cleavage stimulation factor 77 kDa subunit,CstF-77 |
| 6 | G, H, I (C) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) |
Sequence modifications
B: 1 - 572 (UniProt: Q9C0J8)
C: 1 - 244 (UniProt: O95639)
| PDB | External Database | Details |
|---|---|---|
| Met -14 | - | expression tag |
| Gly -13 | - | expression tag |
| Ser -12 | - | expression tag |
| Ser -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| Ser -4 | - | expression tag |
| Ser -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Val 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| His 245 | - | expression tag |
| His 246 | - | expression tag |
| His 247 | - | expression tag |
| His 248 | - | expression tag |
| His 249 | - | expression tag |
| His 250 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 437941.8 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 196.2 | |
| All* | Total formula weight | 438138.1 |
