6UGH

Cryo-EM structure of the apo form of human PRMT5:MEP50 complex at a resolution of 3.4 angstrom

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Protein arginine N-methyltransferase 5	polymer	650	74281.3	1	UniProt (O14744) Pfam (PF17285) Pfam (PF05185) Pfam (PF17286)	Homo sapiens (Human)	72 kDa ICln-binding protein,Histone-arginine N-methyltransferase PRMT5,Jak-binding protein 1,Shk1 kinase-binding protein 1 homolog,SKB1Hs
2	B (B)	Methylosome protein 50	polymer	369	40003.7	1	UniProt (Q9BQA1) Pfam (PF00400)	Homo sapiens (Human)	MEP-50,Androgen receptor cofactor p44,WD repeat-containing protein 77,p44/Mep50

Sequence modifications

A: 1 - 637 (UniProt: O14744)

PDB	External Database	Details
Met -12	-	expression tag
Asp -11	-	expression tag
Tyr -10	-	expression tag
Lys -9	-	expression tag
Asp -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Lys -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Ala -1	-	expression tag
Thr 0	-	expression tag

B: 2 - 342 (UniProt: Q9BQA1)

PDB	External Database	Details
Met -26	-	initiating methionine
Ser -25	-	expression tag
Tyr -24	-	expression tag
Tyr -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Asp -16	-	expression tag
Tyr -15	-	expression tag
Asp -14	-	expression tag
Ile -13	-	expression tag
Pro -12	-	expression tag
Thr -11	-	expression tag
Thr -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	114284.9
All*	Total formula weight	114284.9

*Water molecules are not included.