6U18
Directed evolution of a biosensor selective for the macrolide antibiotic clarithromycin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Erythromycin resistance repressor protein | polymer | 194 | 21602.0 | 2 | UniProt (Q9EVJ6) Pfam (PF00440) | Escherichia coli | Macrolide 2'-phosphotransferase, Macrolide 2'-phosphotransferase MphR(A), MphR(A), MphR(A) erythromycin resistance repressor protein, Regulator of macrolide 2'-phosphotransferase I, Repressor protein MphR(A), TetR/AcrR family transcriptional regulator, Transcriptional regulator, TetR family |
| 2 | C, E (A, B) | CLARITHROMYCIN | non-polymer | 748.0 | 2 | Chemie (CTY) | |||
| 3 | D (A) | CITRATE ANION | non-polymer | 189.1 | 1 | Chemie (FLC) | |||
| 4 | F, G (A, B) | water | water | 18.0 | 135 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 194 (UniProt: Q9EVJ6)
| PDB | External Database | Details |
|---|---|---|
| Thr 122 | Arg 122 | engineered mutation |
| Asn 132 | Lys 132 | engineered mutation |
| Thr 151 | Ala 151 | engineered mutation |
| Gln 184 | His 184 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 43203.9 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 1685.0 | |
| All* | Total formula weight | 44889.0 |
