6TUO
Crystal structure of Archaeoglobus fulgidus Argonaute protein with cognate DNA oligoduplex 5'-pATTGTACGTACAAT
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Piwi protein AF_1318 | polymer | 441 | 50921.1 | 1 | UniProt (O28951) Pfam (PF02171) | Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) | AfAgo,AfPiwi,PIWI/MID domain protein |
| 2 | B, C (R, S) | 5'-D(*AP*TP*TP*GP*TP*AP*CP*GP*TP*AP*CP*AP*AP*TP)-3' | polymer | 14 | 4278.8 | 2 | Escherichia coli | ||
| 3 | D (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 4 | E (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 5 | F, G, H (A) | BETA-MERCAPTOETHANOL | non-polymer | 78.1 | 3 | Chemie (BME) | |||
| 6 | I (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 7 | J, K, L, M (A) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 8 | N, O, P (A, R, S) | water | water | 18.0 | 350 | Chemie (HOH) |
Sequence modifications
A: 1 - 427 (UniProt: O28951)
| PDB | External Database | Details |
|---|---|---|
| Met -13 | - | initiating methionine |
| Gly -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Ser -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Ser -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Pro 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 59478.8 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 768.7 | |
| All* | Total formula weight | 60247.4 |
