6TUO

Crystal structure of Archaeoglobus fulgidus Argonaute protein with cognate DNA oligoduplex 5'-pATTGTACGTACAAT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97970
Spacegroup name	P 2 21 21
Unit cell lengths	52.011, 99.634, 109.802
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.901 - 1.800
R-factor	0.1883
Rwork	0.184
R-free	0.22690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 1ytu
Data reduction software	XDS
Data scaling software	Aimless (0.7.2)
Phasing software	MOLREP (11.6.04; 25.05.2018)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	99.630	99.630	1.840
High resolution limit [Å]	1.800	9.000	1.800
Rmerge	0.044	0.027	0.815
Rmeas	0.048	0.030	0.883
Rpim	0.019	0.013	0.337
Total number of observations		2894	21005
Number of reflections	53664	514	3128
<I/σ(I)>	20.8	60.9	2.6
Completeness [%]	99.9	99	100
Redundancy	6.5	5.6	6.7
CC(1/2)	0.999	0.998	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	280	Na-Cacodylate pH 5.5 0.05 M, KCl_0.2 M, MgCl2 0.01 M, PEG4000 5% (w/v), glycerol 5% (v/v)