6TM5
Cryo-EM structure of the Anaphase-promoting complex/Cyclosome, in complex with the Nek2A substrate at 3.9 angstrom resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Anaphase-promoting complex subunit 1 | polymer | 1944 | 216775.5 | 1 | UniProt (Q9H1A4) Pfam (PF12859) Pfam (PF20518) Pfam (PF21282) Pfam (PF18122) | Homo sapiens (Human) | APC1,Cyclosome subunit 1,Mitotic checkpoint regulator,Testis-specific gene 24 protein |
| 2 | B (B) | Anaphase-promoting complex subunit 11 | polymer | 84 | 9854.6 | 1 | UniProt (Q9NYG5) Pfam (PF12861) | Homo sapiens (Human) | APC11,Cyclosome subunit 11,Hepatocellular carcinoma-associated RING finger protein |
| 3 | C, P (C, P) | Cell division cycle protein 23 homolog | polymer | 597 | 68921.0 | 2 | UniProt (Q9UJX2) Pfam (PF04049) | Homo sapiens (Human) | Anaphase-promoting complex subunit 8,APC8,Cyclosome subunit 8 |
| 4 | D (D) | Anaphase-promoting complex subunit 15 | polymer | 121 | 14286.7 | 1 | UniProt (P60006) Pfam (PF15243) | Homo sapiens (Human) | APC15 |
| 5 | E (E) | Anaphase-promoting complex subunit 16 | polymer | 110 | 11678.0 | 1 | UniProt (Q96DE5) Pfam (PF17256) | Homo sapiens (Human) | APC16,Cyclosome subunit 16 |
| 6 | F, H (F, H) | Cell division cycle protein 27 homolog | polymer | 824 | 91973.1 | 2 | UniProt (P30260) Pfam (PF12895) Pfam (PF00515) | Homo sapiens (Human) | Anaphase-promoting complex subunit 3,APC3,CDC27 homolog,CDC27Hs,H-NUC |
| 7 | G, R (G, W) | Anaphase-promoting complex subunit CDC26 | polymer | 85 | 9794.0 | 2 | UniProt (Q8NHZ8) Pfam (PF10471) | Homo sapiens (Human) | Anaphase-promoting complex subunit 12,APC12,Cell division cycle protein 26 homolog |
| 8 | I (I) | Anaphase-promoting complex subunit 4 | polymer | 808 | 92219.2 | 1 | UniProt (Q9UJX5) Pfam (PF12894) Pfam (PF12896) | Homo sapiens (Human) | APC4,Cyclosome subunit 4 |
| 9 | J, K (J, K) | Cell division cycle protein 16 homolog | polymer | 620 | 71747.5 | 2 | UniProt (Q13042) Pfam (PF12895) Pfam (PF13424) | Homo sapiens (Human) | Anaphase-promoting complex subunit 6,APC6,CDC16 homolog,CDC16Hs,Cyclosome subunit 6 |
| 10 | L (L) | Anaphase-promoting complex subunit 10 | polymer | 185 | 21282.1 | 1 | UniProt (Q9UM13) Pfam (PF03256) | Homo sapiens (Human) | APC10,Cyclosome subunit 10 |
| 11 | M (M) | Anaphase-promoting complex subunit 13 | polymer | 74 | 8528.3 | 1 | UniProt (Q9BS18) Pfam (PF05839) | Homo sapiens (Human) | APC13,Cyclosome subunit 13 |
| 12 | N (N) | Anaphase-promoting complex subunit 2 | polymer | 822 | 93939.0 | 1 | UniProt (Q9UJX6) Pfam (PF00888) Pfam (PF08672) | Homo sapiens (Human) | APC2,Cyclosome subunit 2 |
| 13 | O (O) | Anaphase-promoting complex subunit 5 | polymer | 755 | 85179.8 | 1 | UniProt (Q9UJX4) Pfam (PF21371) Pfam (PF12862) | Homo sapiens (Human) | APC5,Cyclosome subunit 5 |
| 14 | Q (T) | Apc1 loop | polymer | 15 | 1183.3 | 1 | Homo sapiens (Human) | ||
| 15 | S, T (X, Y) | Anaphase-promoting complex subunit 7 | polymer | 599 | 66929.4 | 2 | UniProt (Q9UJX3) Pfam (PF13181) Pfam (PF13432) | Homo sapiens (Human) | APC7,Cyclosome subunit 7 |
| 16 | U (S) | Serine/threonine-protein kinase Nek2 | polymer | 445 | 51788.4 | 1 | UniProt (P51955) Pfam (PF00069) | Homo sapiens (Human) | HSPK 21,Never in mitosis A-related kinase 2,NimA-related protein kinase 2,NimA-like protein kinase 1 |
| 17 | V (Q) | Serine/threonine-protein kinase Nek2 | polymer | 445 | 51848.6 | 1 | UniProt (P51955) Pfam (PF00069) | Homo sapiens (Human) | HSPK 21,Never in mitosis A-related kinase 2,NimA-related protein kinase 2,NimA-like protein kinase 1 |
| 18 | W, X, Y (B) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) |
Sequence modifications
A: 1 - 1944 (UniProt: Q9H1A4)
S: 55 - 499 (UniProt: P51955)
| PDB | External Database | Details |
|---|---|---|
| Phe 356 | Pro 356 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ala 449 | Met 395 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 22 |
| Total formula weight | 1277293.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 196.2 | |
| All* | Total formula weight | 1277489.9 |
