6TKO

Phosphorylated turkey beta1 adrenoceptor with bound agonist formoterol coupled to arrestin-2 in lipid nanodisc.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Beta-1 adrenergic receptor	polymer	328	37275.4	1	UniProt (P07700) Pfam (PF00001)	Meleagris gallopavo (Wild turkey)	Beta-1 adrenoreceptor,Beta-T
2	B (B)	Beta-arrestin-1	polymer	418	47021.3	1	UniProt (P49407) Pfam (PF00339) Pfam (PF02752)	Homo sapiens (Human)	Arrestin beta-1,Non-visual arrestin-2
3	C (H)	Fab30 heavy chain	polymer	237	25512.4	1		Phage display vector pTDisp
4	D (L)	Fab30 light chain	polymer	215	23435.1	1		Phage display vector pTDisp
5	E (A)	~{N}-[5-[(1~{R})-2-[[(2~{R})-1-(4-methoxyphenyl)propan-2-yl]amino]-1-oxidanyl-ethyl]-2-oxidanyl-phenyl]methanamide	non-polymer		344.4	1	Chemie (H98)

Sequence modifications

A: 32 - 357 (UniProt: P07700)

PDB	External Database	Details
Gly 32	Ser 32	engineered mutation
Cys 44	Met 44	engineered mutation
Val 90	Met 90	engineered mutation
Cys 103	Val 103	engineered mutation
Leu 116	Cys 116	engineered mutation
Trp 130	Glu 130	engineered mutation
Lys 322	Asp 322	engineered mutation
Ala 327	Phe 327	engineered mutation
Met 338	Phe 338	engineered mutation
Ala 358	-	expression tag
Ala 1343	-	expression tag
Arg 1344	-	expression tag
Gly 1345	-	expression tag
Arg 1346	-	expression tag
Pro 1347	-	expression tag
Leu 1348	-	expression tag
Pro 1349	-	expression tag
Glu 1350	-	expression tag
Thr 1351	-	expression tag
Gly 1352	-	expression tag
Gly 1353	-	expression tag
Gly 1354	-	expression tag
Asp 1355	-	expression tag
Glu 1356	-	expression tag
Sep 1357	-	expression tag
Ala 1358	-	expression tag
Tpo 1359	-	expression tag
Tpo 1360	-	expression tag
Ala 1361	-	expression tag
Sep 1362	-	expression tag
Sep 1363	-	expression tag
Sep 1364	-	expression tag
Leu 1365	-	expression tag
Ala 1366	-	expression tag
Lys 1367	-	expression tag
Asp 1368	-	expression tag
Thr 1369	-	expression tag
Ser 1370	-	expression tag
Ser 1371	-	expression tag

B: 1 - 418 (UniProt: P49407)

PDB	External Database	Details
Gly 1	Met 1	engineered mutation
Cys 68	Leu 68	engineered mutation
Glu 169	Arg 169	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	133244.1
Non-Polymers*	Number of molecules	1
	Total formula weight	344.4
All*	Total formula weight	133588.6

*Water molecules are not included.