6TC3
Cryo-EM structure of an Escherichia coli ribosome-SpeFL complex stalled in response to L-ornithine (Replicate 1)
This is a non-PDB format compatible entry.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | 16S1 | 16S rRNA | polymer | 1534 | 497405.0 | 1 | GenBank (1108560344) | Escherichia coli K-12 | |
2 | S021 | 30S ribosomal protein S2 | polymer | 241 | 26781.7 | 1 | UniProt (P0A7V0) Pfam (PF00318) In PDB | Escherichia coli K-12 | Small ribosomal subunit protein uS2 |
3 | S031 | 30S ribosomal protein S3 | polymer | 233 | 26031.3 | 1 | UniProt (P0A7V3) Pfam (PF07650) Pfam (PF00189) In PDB | Escherichia coli K-12 | Small ribosomal subunit protein uS3 |
4 | S041 | 30S ribosomal protein S4 | polymer | 206 | 23514.2 | 1 | UniProt (P0A7V8) Pfam (PF00163) Pfam (PF01479) In PDB | Escherichia coli K-12 | Small ribosomal subunit protein uS4 |
5 | S051 | 30S ribosomal protein S5 | polymer | 167 | 17629.4 | 1 | UniProt (P0A7W1) Pfam (PF00333) Pfam (PF03719) In PDB | Escherichia coli K-12 | Small ribosomal subunit protein uS5 |
6 | S061 | 30S ribosomal protein S6 | polymer | 135 | 15727.5 | 1 | UniProt (P02358) Pfam (PF01250) In PDB | Escherichia coli K-12 | Small ribosomal subunit protein bS6 |
7 | S071 | 30S ribosomal protein S7 | polymer | 179 | 20055.2 | 1 | UniProt (P02359) Pfam (PF00177) In PDB | Escherichia coli K-12 | Small ribosomal subunit protein uS7 |
8 | S081 | 30S ribosomal protein S8 | polymer | 130 | 14146.6 | 1 | UniProt (P0A7W7) Pfam (PF00410) In PDB | Escherichia coli K-12 | Small ribosomal subunit protein uS8 |
9 | S091 | 30S ribosomal protein S9 | polymer | 130 | 14886.3 | 1 | UniProt (P0A7X3) Pfam (PF00380) In PDB | Escherichia coli K-12 | Small ribosomal subunit protein uS9 |
10 | S101 | 30S ribosomal protein S10 | polymer | 103 | 11755.6 | 1 | UniProt (P0A7R5) Pfam (PF00338) In PDB | Escherichia coli K-12 | Small ribosomal subunit protein uS10 |
11 | S111 | 30S ribosomal protein S11 | polymer | 129 | 13871.0 | 1 | UniProt (P0A7R9) Pfam (PF00411) In PDB | Escherichia coli K-12 | Small ribosomal subunit protein uS11 |
12 | S121 | 30S ribosomal protein S12 | polymer | 124 | 13814.2 | 1 | UniProt (P0A7S3) Pfam (PF00164) In PDB | Escherichia coli K-12 | Small ribosomal subunit protein uS12 |
13 | S131 | 30S ribosomal protein S13 | polymer | 118 | 13128.5 | 1 | UniProt (P0A7S9) Pfam (PF00416) In PDB | Escherichia coli K-12 | Small ribosomal subunit protein uS13 |
14 | S141 | 30S ribosomal protein S14 | polymer | 102 | 11677.6 | 1 | UniProt (P0AG59) Pfam (PF00253) In PDB | Escherichia coli K-12 | Small ribosomal subunit protein uS14 |
15 | S151 | 30S ribosomal protein S15 | polymer | 89 | 10290.8 | 1 | UniProt (P0ADZ4) Pfam (PF00312) In PDB | Escherichia coli K-12 | Small ribosomal subunit protein uS15 |
16 | S161 | 30S ribosomal protein S16 | polymer | 82 | 9207.6 | 1 | UniProt (P0A7T3) Pfam (PF00886) In PDB | Escherichia coli K-12 | Small ribosomal subunit protein bS16 |
17 | S171 | 30S ribosomal protein S17 | polymer | 84 | 9724.5 | 1 | UniProt (P0AG63) Pfam (PF00366) In PDB | Escherichia coli K-12 | Small ribosomal subunit protein uS17 |
18 | S181 | 30S ribosomal protein S18 | polymer | 75 | 9005.5 | 1 | UniProt (P0A7T7) Pfam (PF01084) In PDB | Escherichia coli K-12 | Small ribosomal subunit protein bS18 |
19 | S191 | 30S ribosomal protein S19 | polymer | 92 | 10455.4 | 1 | UniProt (P0A7U3) Pfam (PF00203) In PDB | Escherichia coli K-12 | Small ribosomal subunit protein uS19 |
20 | S201 | 30S ribosomal protein S20 | polymer | 87 | 9708.5 | 1 | UniProt (P0A7U7) Pfam (PF01649) In PDB | Escherichia coli K-12 | Small ribosomal subunit protein bS20 |
21 | S211 | 30S ribosomal protein S21 | polymer | 71 | 8524.0 | 1 | UniProt (P68679) Pfam (PF01165) In PDB | Escherichia coli K-12 | Small ribosomal subunit protein bS21 |
22 | 23S1 | 23S rRNA | polymer | 2897 | 939607.3 | 1 | Escherichia coli K-12 | ||
23 | 05S1 | 5S rRNA | polymer | 120 | 38790.1 | 1 | GenBank (1726050194) | Escherichia coli K-12 | |
24 | L021 | 50S ribosomal protein L2 | polymer | 273 | 29923.6 | 1 | UniProt (P60422) Pfam (PF00181) Pfam (PF03947) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein uL2 |
25 | L031 | 50S ribosomal protein L3 | polymer | 209 | 22291.6 | 1 | UniProt (P60438) Pfam (PF00297) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein uL3 |
26 | L041 | 50S ribosomal protein L4 | polymer | 201 | 22121.6 | 1 | UniProt (P60723) Pfam (PF00573) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein uL4 |
27 | L051 | 50S ribosomal protein L5 | polymer | 179 | 20333.6 | 1 | UniProt (P62399) Pfam (PF00281) Pfam (PF00673) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein uL5 |
28 | L061 | 50S ribosomal protein L6 | polymer | 177 | 18932.8 | 1 | UniProt (P0AG55) Pfam (PF00347) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein uL6 |
29 | L091 | 50S ribosomal protein L9 | polymer | 149 | 15789.0 | 1 | UniProt (P0A7R1) Pfam (PF03948) Pfam (PF01281) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein bL9 |
30 | L311 | 50S ribosomal protein L31 | polymer | 70 | 7887.1 | 1 | UniProt (P0A7M9) Pfam (PF01197) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein bL31-A |
31 | L131 | 50S ribosomal protein L13 | polymer | 142 | 16050.6 | 1 | UniProt (P0AA10) Pfam (PF00572) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein uL13 |
32 | L141 | 50S ribosomal protein L14 | polymer | 123 | 13565.1 | 1 | UniProt (P0ADY3) Pfam (PF00238) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein uL14 |
33 | L151 | 50S ribosomal protein L15 | polymer | 144 | 15008.5 | 1 | UniProt (P02413) Pfam (PF00828) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein uL15 |
34 | L161 | 50S ribosomal protein L16 | polymer | 136 | 15327.3 | 1 | UniProt (P0ADY7) Pfam (PF00252) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein uL16 |
35 | L171 | 50S ribosomal protein L17 | polymer | 127 | 14393.7 | 1 | UniProt (P0AG44) Pfam (PF01196) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein bL17 |
36 | L181 | 50S ribosomal protein L18 | polymer | 117 | 12794.7 | 1 | UniProt (P0C018) Pfam (PF00861) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein uL18 |
37 | L191 | 50S ribosomal protein L19 | polymer | 115 | 13159.3 | 1 | UniProt (P0A7K6) Pfam (PF01245) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein bL19 |
38 | L201 | 50S ribosomal protein L20 | polymer | 118 | 13528.0 | 1 | UniProt (P0A7L3) Pfam (PF00453) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein bL20 |
39 | L211 | 50S ribosomal protein L21 | polymer | 103 | 11586.4 | 1 | UniProt (P0AG48) Pfam (PF00829) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein bL21 |
40 | L221 | 50S ribosomal protein L22 | polymer | 110 | 12253.4 | 1 | UniProt (P61175) Pfam (PF00237) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein uL22 |
41 | L231 | 50S ribosomal protein L23 | polymer | 100 | 11222.2 | 1 | UniProt (P0ADZ0) Pfam (PF00276) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein uL23 |
42 | L241 | 50S ribosomal protein L24 | polymer | 104 | 11339.3 | 1 | UniProt (P60624) Pfam (PF00467) Pfam (PF17136) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein uL24 |
43 | L251 | 50S ribosomal protein L25 | polymer | 94 | 10713.5 | 1 | UniProt (P68919) Pfam (PF01386) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein bL25 |
44 | L271 | 50S ribosomal protein L27 | polymer | 85 | 9146.5 | 1 | UniProt (P0A7L8) Pfam (PF01016) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein bL27 |
45 | L281 | 50S ribosomal protein L28 | polymer | 78 | 9027.6 | 1 | UniProt (P0A7M2) Pfam (PF00830) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein bL28 |
46 | L291 | 50S ribosomal protein L29 | polymer | 63 | 7286.5 | 1 | UniProt (P0A7M6) Pfam (PF00831) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein uL29 |
47 | L301 | 50S ribosomal protein L30 | polymer | 59 | 6554.8 | 1 | UniProt (P0AG51) Pfam (PF00327) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein uL30 |
48 | L321 | 50S ribosomal protein L32 | polymer | 57 | 6463.4 | 1 | UniProt (P0A7N4) Pfam (PF01783) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein bL32 |
49 | L331 | 50S ribosomal protein L33 | polymer | 55 | 6388.6 | 1 | UniProt (P0A7N9) Pfam (PF00471) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein bL33 |
50 | L341 | 50S ribosomal protein L34 | polymer | 46 | 5397.5 | 1 | UniProt (P0A7P5) Pfam (PF00468) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein bL34 |
51 | L351 | 50S ribosomal protein L35 | polymer | 65 | 7313.0 | 1 | UniProt (P0A7Q1) Pfam (PF01632) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein bL35,Ribosomal protein A |
52 | L361 | 50S ribosomal protein L36 | polymer | 38 | 4377.4 | 1 | UniProt (P0A7Q6) Pfam (PF00444) In PDB | Escherichia coli K-12 | Large ribosomal subunit protein bL36-A,Ribosomal protein B |
53 | SPE1 | SpeFL | polymer | 34 | 4286.9 | 1 | UniProt (A0A4S4NWS2) UniProt (by SIFTS) (P0DTV7) In PDB | Escherichia coli K-12 | |
54 | MRN1 | mRNA | polymer | 7 | 2173.3 | 1 | Escherichia coli K-12 | ||
55 | PTR1 | P-site Arg-tRNA | polymer | 76 | 24582.8 | 1 | Escherichia coli K-12 | ||
56 | 16S1, 23S1, PTR1, L231, SPE1 | MAGNESIUM ION | non-polymer | 24.3 | 339 | Chemie (MG) | |||
57 | 16S1, 23S1, 05S1, L031 | POTASSIUM ION | non-polymer | 39.1 | 147 | Chemie (K) | |||
58 | 23S1, 16S1, L021, L031, L171... | UNKNOWN ATOM OR ION | non-polymer | 35.5 | 1201 | Chemie (UNX) | |||
59 | L311, S021, L361 | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) | |||
60 | 23S1 | L-ornithine | non-polymer | 132.2 | 1 | Chemie (ORN) | |||
61 | water | water | 18.0 | 800 | Chemie (HOH) |
Sequence modifications
S141: 0 - 101 (UniProt: P0AG59)
SPE1: 1 - 34 (UniProt: A0A4S4NWS2)
PDB | External Database | Details |
---|---|---|
Ala 35 | - | insertion |
PDB | External Database | Details |
---|---|---|
Ser 5 | Asn 5 | conflict |
Thr 7 | Leu 7 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 55 |
Total formula weight | 2166956.8 | |
Non-Polymers* | Number of molecules | 1691 |
Total formula weight | 56894.2 | |
All* | Total formula weight | 2223851.1 |