6TC3
Cryo-EM structure of an Escherichia coli ribosome-SpeFL complex stalled in response to L-ornithine (Replicate 1)
This is a non-PDB format compatible entry.
Summary for 6TC3
| Entry DOI | 10.2210/pdb6tc3/pdb |
| EMDB information | 10458 |
| Descriptor | 16S rRNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... (61 entities in total) |
| Functional Keywords | ribosome, spefl, arrest peptide, ornithine, protein synthesis |
| Biological source | Escherichia coli K-12 More |
| Total number of polymer chains | 55 |
| Total formula weight | 2223851.05 |
| Authors | Innis, C.A.,Herrero del Valle, A. (deposition date: 2019-11-05, release date: 2020-01-01, Last modification date: 2025-03-12) |
| Primary citation | Herrero Del Valle, A.,Seip, B.,Cervera-Marzal, I.,Sacheau, G.,Seefeldt, A.C.,Innis, C.A. Ornithine capture by a translating ribosome controls bacterial polyamine synthesis. Nat Microbiol, 5:554-561, 2020 Cited by PubMed Abstract: Polyamines are essential metabolites that play an important role in cell growth, stress adaptation and microbial virulence. To survive and multiply within a human host, pathogenic bacteria adjust the expression and activity of polyamine biosynthetic enzymes in response to different environmental stresses and metabolic cues. Here, we show that ornithine capture by the ribosome and the nascent peptide SpeFL controls polyamine synthesis in γ-proteobacteria by inducing the expression of the ornithine decarboxylase SpeF, via a mechanism involving ribosome stalling and transcription antitermination. In addition, we present the cryogenic electron microscopy structure of an Escherichia coli ribosome stalled during translation of speFL in the presence of ornithine. The structure shows how the ribosome and the SpeFL sensor domain form a highly selective binding pocket that accommodates a single ornithine molecule but excludes near-cognate ligands. Ornithine pre-associates with the ribosome and is then held in place by the sensor domain, leading to the compaction of the SpeFL effector domain and blocking the action of release factor 1. Thus, our study not only reveals basic strategies by which nascent peptides assist the ribosome in detecting a specific metabolite, but also provides a framework for assessing how ornithine promotes virulence in several human pathogens. PubMed: 32094585DOI: 10.1038/s41564-020-0669-1 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.7 Å) |
Structure validation
Download full validation report
