6TBM

Structure of SAGA bound to TBP, including Spt8 and DUB

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	M	TATA-box Binding Protein (TBP)	polymer	240	27042.3	1	Pfam (PF00352) UniProt (by SIFTS) (P13393) In PDB	Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast)
2	A	Transcriptional coactivator HFI1/ADA1	polymer	448	49888.3	1	UniProt (C4QZA3) Pfam (PF12767) In PDB	Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast)
3	C	Subunit of SAGA histone acetyltransferase complex	polymer	698	79995.2	1	UniProt (C4QZ39) Pfam (PF08313) In PDB	Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast Pichia pastoris)
4	F	Spt20	polymer	517	59530.6	1	UniProt (C4QZ05) Pfam (PF12090) In PDB	Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast)
5	D	Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to act	polymer	341	39030.1	1	UniProt (C4R3E4) Pfam (PF02269) In PDB	Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast)
6	E	Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex	polymer	1191	136768.8	1	UniProt (C4R5C7) Pfam (PF00439) In PDB	Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast)
7	J	Transcription initiation factor TFIID subunit 10	polymer	217	23879.1	1	UniProt (C4QXP2) Pfam (PF03540) In PDB	Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast)
8	K	Subunit (61/68 kDa) of TFIID and SAGA complexes	polymer	609	66690.1	1	UniProt (C4R150) Pfam (PF03847) In PDB	Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast)
9	G	Subunit (90 kDa) of TFIID and SAGA complexes	polymer	722	80933.0	1	UniProt (C4R4L4) Pfam (PF00400) Pfam (PF08513) Pfam (PF04494) In PDB	Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast)
10	H	Subunit (60 kDa) of TFIID and SAGA complexes	polymer	485	54888.4	1	UniProt (C4QW33) Pfam (PF02969) Pfam (PF07571) In PDB	Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast)
11	I	Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation	polymer	153	17303.6	1	UniProt (C4QZS5) Pfam (PF02291) In PDB	Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast)
12	L	Transcription-associated protein	polymer	3825	438055.3	1	UniProt (C4QYV4) Pfam (PF20175) Pfam (PF20206) Pfam (PF02259) Pfam (PF00454) In PDB	Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast)
13	B	Transcriptional regulator involved in glucose repression of Gal4p-regulated genes	polymer	722	81864.3	1	UniProt (C4R2A4) Pfam (PF10198) In PDB	Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast)
14	N	Spt8	polymer	400	34059.9	1		Komagataella phaffii GS115 (Yeast)
15	R	Polyubiquitin-B	polymer	76	8560.8	1	UniProt (J3QS39) Pfam (PF00240) UniProt (by SIFTS) (P0CG47) In PDB	Homo sapiens (Human)
16	Q	Ubiquitin carboxyl-terminal hydrolase	polymer	502	56745.9	1	UniProt (C4QY61) Pfam (PF02148) Pfam (PF00443) In PDB	Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast)
17	O	SAGA-associated factor 11	polymer	123	13977.7	1	UniProt (C4R2D9) Pfam (PF08209) In PDB	Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast)
18	P	Transcription and mRNA export factor SUS1	polymer	96	11126.7	1	UniProt (C4R1P1) Pfam (PF10163) In PDB	Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast)
19		water	water		18.0	91	Chemie (HOH)

Sequence modifications

C: -8 - 644 (UniProt: C4QZ39)

PDB	External Database	Details
Leu 645	-	expression tag
Glu 646	-	expression tag
Gly 647	-	expression tag
Gly 648	-	expression tag
Gly 649	-	expression tag
Gly 650	-	expression tag
Ser 651	-	expression tag
Met 652	-	expression tag
Asp 653	-	expression tag
Glu 654	-	expression tag
Lys 655	-	expression tag
Thr 656	-	expression tag
Thr 657	-	expression tag
Gly 658	-	expression tag
Trp 659	-	expression tag
Arg 660	-	expression tag
Gly 661	-	expression tag
Gly 662	-	expression tag
His 663	-	expression tag
Val 664	-	expression tag
Val 665	-	expression tag
Glu 666	-	expression tag
Gly 667	-	expression tag
Leu 668	-	expression tag
Ala 669	-	expression tag
Gly 670	-	expression tag
Glu 671	-	expression tag
Leu 672	-	expression tag
Glu 673	-	expression tag
Gln 674	-	expression tag
Leu 675	-	expression tag
Arg 676	-	expression tag
Ala 677	-	expression tag
Arg 678	-	expression tag
Leu 679	-	expression tag
Glu 680	-	expression tag
His 681	-	expression tag
His 682	-	expression tag
Pro 683	-	expression tag
Gln 684	-	expression tag
Gly 685	-	expression tag
Gln 686	-	expression tag
Arg 687	-	expression tag
Glu 688	-	expression tag
Pro 689	-	expression tag
Gly 690	-	expression tag
Gly 691	-	expression tag
Ser 692	-	expression tag
His 693	-	expression tag
His 694	-	expression tag
His 695	-	expression tag
His 696	-	expression tag
His 697	-	expression tag
His 698	-	expression tag

R: 1 - 75 (UniProt: J3QS39)

PDB	External Database	Details
Glz 76	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	18
	Total formula weight	1280340.3
All*	Total formula weight	1280340.3

*Water molecules are not included.