6T5T

Crystal structure of Archaeoglobus fulgidus Argonaute protein with cognate DNA oligoduplex 5'-pATTGTGGCCACAAT

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Piwi protein AF_1318	polymer	441	50921.1	1	UniProt (O28951) Pfam (PF02171) In PDB	Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)	AfAgo,AfPiwi,PIWI/MID domain protein
2	R, S	5'-D(*AP*TP*TP*GP*TP*GP*GP*CP*CP*AP*CP*AP*AP*TP)-3'	polymer	14	4279.8	2		Escherichia coli
3	A	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
4	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5	A	POTASSIUM ION	non-polymer		39.1	3	Chemie (K)
6	A	BETA-MERCAPTOETHANOL	non-polymer		78.1	6	Chemie (BME)
7		water	water		18.0	318	Chemie (HOH)

Sequence modifications

A: 1 - 427 (UniProt: O28951)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	59480.7
Non-Polymers*	Number of molecules	13
	Total formula weight	694.5
All*	Total formula weight	60175.2

*Water molecules are not included.