6T5T

Crystal structure of Archaeoglobus fulgidus Argonaute protein with cognate DNA oligoduplex 5'-pATTGTGGCCACAAT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97970
Spacegroup name	P 2 21 21
Unit cell lengths	52.098, 99.551, 109.901
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.951 - 1.700
Rwork	0.180
R-free	0.21340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 1ytu
Data reduction software	XDS (VERSION Jan 26, 2018 BUILT=20180126)
Data scaling software	SCALA (3.3.22)
Phasing software	MOLREP (Vers 11.6.04; 25.05.2018)
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	99.551	99.551	1.790
High resolution limit [Å]	1.700	5.380	1.700
Rmerge		0.047	0.969
Rmeas	0.068	0.052	1.075
Rpim	0.021	0.016	0.335
Total number of observations	657679	20531	93225
Number of reflections	63695	2222	9192
<I/σ(I)>	20.8	61.1	2.3
Completeness [%]	100.0	99.8	100
Redundancy	10.3	9.2	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	280	Na-Cacodylate pH 5.5 0.05 M, KCl_0.2 M, MgCl2 0.01 M, PEG4000 5% (w/v), glycerol 5% (v/v)