6T22

N-terminal domain of EcoKMcrA restriction endonuclease (NEco) in complex with T5hmCGA target sequence

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	EcoKMcrA modification dependent restriction endonuclease	polymer	152	17368.6	2	UniProt (P24200) In PDB	Escherichia coli K-12
2	C, E	DNA (5'-D(*TP*CP*AP*TP*(5HC)P*GP*AP*TP*TP*C)-3')	polymer	10	3025.0	2		synthetic construct
3	D, F	DNA (5'-D(*GP*AP*AP*TP*(5HC)P*GP*AP*TP*GP*A)-3')	polymer	10	3123.1	2		synthetic construct
4		water	water		18.0	405	Chemie (HOH)

Sequence modifications

A, B: 1 - 143 (UniProt: P24200)

PDB	External Database	Details
Gly -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	47033.4
All*	Total formula weight	47033.4

*Water molecules are not included.