6SKL
Cryo-EM structure of the CMG Fork Protection Complex at a replication fork - Conformation 1
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (2) | DNA replication licensing factor MCM2 | polymer | 868 | 98911.5 | 1 | UniProt (P29469) Pfam (PF12619) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | Minichromosome maintenance protein 2 |
| 2 | B (3) | DNA replication licensing factor MCM3 | polymer | 971 | 107653.5 | 1 | UniProt (P24279) Pfam (PF22207) Pfam (PF23191) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | Minichromosome maintenance protein 3 |
| 3 | C (4) | DNA replication licensing factor MCM4 | polymer | 933 | 105138.4 | 1 | UniProt (P30665) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21128) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | Cell division control protein 54 |
| 4 | D (5) | Minichromosome maintenance protein 5 | polymer | 775 | 86505.7 | 1 | UniProt (P29496) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21933) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | Cell division control protein 46 |
| 5 | E (6) | DNA replication licensing factor MCM6 | polymer | 1017 | 113110.2 | 1 | UniProt (P53091) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF18263) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | Minichromosome maintenance protein 6 |
| 6 | F (7) | DNA replication licensing factor MCM7 | polymer | 845 | 95049.9 | 1 | UniProt (P38132) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF24901) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | Cell division control protein 47,Minichromosome maintenance protein 7 |
| 7 | G (A) | DNA replication complex GINS protein PSF1 | polymer | 208 | 24230.6 | 1 | UniProt (Q12488) Pfam (PF05916) Pfam (PF24997) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | Partner of Sld five 1 |
| 8 | H (B) | DNA replication complex GINS protein PSF2 | polymer | 213 | 25096.8 | 1 | UniProt (P40359) Pfam (PF25005) Pfam (PF05916) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | Partner of Sld five 2 |
| 9 | I (C) | DNA replication complex GINS protein PSF3 | polymer | 217 | 24437.9 | 1 | UniProt (Q12146) Pfam (PF22466) Pfam (PF05916) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | Partner of Sld five 3 |
| 10 | J (D) | DNA replication complex GINS protein SLD5 | polymer | 294 | 33983.6 | 1 | UniProt (Q03406) Pfam (PF05916) Pfam (PF16922) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | |
| 11 | K (E) | Cell division control protein 45 | polymer | 650 | 74324.8 | 1 | UniProt (Q08032) Pfam (PF02724) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | |
| 12 | L, M, N (F, G, H) | DNA polymerase alpha-binding protein | polymer | 927 | 104543.4 | 3 | UniProt (Q01454) Pfam (PF12341) Pfam (PF20946) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | Chromosome replication protein CHL15,Chromosome transmission fidelity protein 4,Protein POB1 |
| 13 | O (I) | DNA fork, leading-strand template | polymer | 85 | 26396.8 | 1 | synthetic construct | ||
| 14 | P (J) | DNA fork, lagging-strand template | polymer | 61 | 18524.9 | 1 | synthetic construct | ||
| 15 | Q (X) | Topoisomerase 1-associated factor 1 | polymer | 1238 | 141296.9 | 1 | UniProt (P53840) Pfam (PF04821) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | |
| 16 | R (Y) | Chromosome segregation in meiosis protein 3 | polymer | 317 | 36402.6 | 1 | UniProt (Q04659) Pfam (PF07962) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | |
| 17 | AA, BA, CA, S, X (5, 6, 7, 2, 4) | ZINC ION | non-polymer | 65.4 | 5 | Chemie (ZN) | |||
| 18 | T, V, Y (2, 3, 5) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 3 | Chemie (ANP) | |||
| 19 | U, W, Z (2, 3, 5) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) |
Sequence modifications
C: 1 - 194 (UniProt: Q12146)
| PDB | External Database | Details |
|---|---|---|
| Met -22 | - | initiating methionine |
| Gly -21 | - | expression tag |
| Ser -20 | - | expression tag |
| Ser -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| Ser -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Gly -10 | - | expression tag |
| Leu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Pro -7 | - | expression tag |
| Arg -6 | - | expression tag |
| Gly -5 | - | expression tag |
| Ser -4 | - | expression tag |
| His -3 | - | expression tag |
| Met -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 18 |
| Total formula weight | 1324694.3 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 1918.5 | |
| All* | Total formula weight | 1326612.8 |
