Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6SKL

Cryo-EM structure of the CMG Fork Protection Complex at a replication fork - Conformation 1

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
2	0000727	biological_process	double-strand break repair via break-induced replication
2	0003677	molecular_function	DNA binding
2	0003682	molecular_function	chromatin binding
2	0003688	molecular_function	DNA replication origin binding
2	0003697	molecular_function	single-stranded DNA binding
2	0004386	molecular_function	helicase activity
2	0005515	molecular_function	protein binding
2	0005524	molecular_function	ATP binding
2	0005634	cellular_component	nucleus
2	0005654	cellular_component	nucleoplasm
2	0005656	cellular_component	nuclear pre-replicative complex
2	0005737	cellular_component	cytoplasm
2	0006260	biological_process	DNA replication
2	0006267	biological_process	pre-replicative complex assembly involved in nuclear cell cycle DNA replication
2	0006268	biological_process	DNA unwinding involved in DNA replication
2	0006270	biological_process	DNA replication initiation
2	0006271	biological_process	DNA strand elongation involved in DNA replication
2	0006279	biological_process	premeiotic DNA replication
2	0006974	biological_process	DNA damage response
2	0008270	molecular_function	zinc ion binding
2	0016787	molecular_function	hydrolase activity
2	0016887	molecular_function	ATP hydrolysis activity
2	0017116	molecular_function	single-stranded DNA helicase activity
2	0031261	cellular_component	DNA replication preinitiation complex
2	0031298	cellular_component	replication fork protection complex
2	0032508	biological_process	DNA duplex unwinding
2	0042555	cellular_component	MCM complex
2	0043138	molecular_function	3'-5' DNA helicase activity
2	0043596	cellular_component	nuclear replication fork
2	0046872	molecular_function	metal ion binding
2	0071162	cellular_component	CMG complex
2	1902975	biological_process	mitotic DNA replication initiation
2	1905775	biological_process	negative regulation of DNA helicase activity
3	0000727	biological_process	double-strand break repair via break-induced replication
3	0000781	cellular_component	chromosome, telomeric region
3	0003677	molecular_function	DNA binding
3	0003682	molecular_function	chromatin binding
3	0003688	molecular_function	DNA replication origin binding
3	0003697	molecular_function	single-stranded DNA binding
3	0004386	molecular_function	helicase activity
3	0005515	molecular_function	protein binding
3	0005524	molecular_function	ATP binding
3	0005634	cellular_component	nucleus
3	0005654	cellular_component	nucleoplasm
3	0005656	cellular_component	nuclear pre-replicative complex
3	0005737	cellular_component	cytoplasm
3	0006260	biological_process	DNA replication
3	0006267	biological_process	pre-replicative complex assembly involved in nuclear cell cycle DNA replication
3	0006268	biological_process	DNA unwinding involved in DNA replication
3	0006270	biological_process	DNA replication initiation
3	0006271	biological_process	DNA strand elongation involved in DNA replication
3	0006279	biological_process	premeiotic DNA replication
3	0016787	molecular_function	hydrolase activity
3	0016887	molecular_function	ATP hydrolysis activity
3	0017116	molecular_function	single-stranded DNA helicase activity
3	0030466	biological_process	silent mating-type cassette heterochromatin formation
3	0031261	cellular_component	DNA replication preinitiation complex
3	0031298	cellular_component	replication fork protection complex
3	0031509	biological_process	subtelomeric heterochromatin formation
3	0032508	biological_process	DNA duplex unwinding
3	0042555	cellular_component	MCM complex
3	0043596	cellular_component	nuclear replication fork
3	0071162	cellular_component	CMG complex
3	1902975	biological_process	mitotic DNA replication initiation
3	1904931	molecular_function	MCM complex binding
4	0000727	biological_process	double-strand break repair via break-induced replication
4	0003677	molecular_function	DNA binding
4	0003678	molecular_function	DNA helicase activity
4	0003688	molecular_function	DNA replication origin binding
4	0003697	molecular_function	single-stranded DNA binding
4	0004386	molecular_function	helicase activity
4	0005515	molecular_function	protein binding
4	0005524	molecular_function	ATP binding
4	0005634	cellular_component	nucleus
4	0005654	cellular_component	nucleoplasm
4	0005656	cellular_component	nuclear pre-replicative complex
4	0005737	cellular_component	cytoplasm
4	0006260	biological_process	DNA replication
4	0006267	biological_process	pre-replicative complex assembly involved in nuclear cell cycle DNA replication
4	0006268	biological_process	DNA unwinding involved in DNA replication
4	0006270	biological_process	DNA replication initiation
4	0006271	biological_process	DNA strand elongation involved in DNA replication
4	0006279	biological_process	premeiotic DNA replication
4	0008270	molecular_function	zinc ion binding
4	0009378	molecular_function	four-way junction helicase activity
4	0016787	molecular_function	hydrolase activity
4	0016887	molecular_function	ATP hydrolysis activity
4	0017116	molecular_function	single-stranded DNA helicase activity
4	0031261	cellular_component	DNA replication preinitiation complex
4	0031298	cellular_component	replication fork protection complex
4	0032508	biological_process	DNA duplex unwinding
4	0033260	biological_process	nuclear DNA replication
4	0042555	cellular_component	MCM complex
4	0043138	molecular_function	3'-5' DNA helicase activity
4	0043596	cellular_component	nuclear replication fork
4	0071162	cellular_component	CMG complex
4	0097373	cellular_component	MCM core complex
4	1902975	biological_process	mitotic DNA replication initiation
5	0000727	biological_process	double-strand break repair via break-induced replication
5	0000781	cellular_component	chromosome, telomeric region
5	0003677	molecular_function	DNA binding
5	0003682	molecular_function	chromatin binding
5	0003688	molecular_function	DNA replication origin binding
5	0003697	molecular_function	single-stranded DNA binding
5	0004386	molecular_function	helicase activity
5	0005515	molecular_function	protein binding
5	0005524	molecular_function	ATP binding
5	0005634	cellular_component	nucleus
5	0005654	cellular_component	nucleoplasm
5	0005656	cellular_component	nuclear pre-replicative complex
5	0005737	cellular_component	cytoplasm
5	0006260	biological_process	DNA replication
5	0006267	biological_process	pre-replicative complex assembly involved in nuclear cell cycle DNA replication
5	0006268	biological_process	DNA unwinding involved in DNA replication
5	0006270	biological_process	DNA replication initiation
5	0006279	biological_process	premeiotic DNA replication
5	0006368	biological_process	transcription elongation by RNA polymerase II
5	0008270	molecular_function	zinc ion binding
5	0016787	molecular_function	hydrolase activity
5	0016887	molecular_function	ATP hydrolysis activity
5	0017116	molecular_function	single-stranded DNA helicase activity
5	0030174	biological_process	regulation of DNA-templated DNA replication initiation
5	0031261	cellular_component	DNA replication preinitiation complex
5	0031298	cellular_component	replication fork protection complex
5	0031507	biological_process	heterochromatin formation
5	0031509	biological_process	subtelomeric heterochromatin formation
5	0032508	biological_process	DNA duplex unwinding
5	0033260	biological_process	nuclear DNA replication
5	0042555	cellular_component	MCM complex
5	0043138	molecular_function	3'-5' DNA helicase activity
5	0043596	cellular_component	nuclear replication fork
5	0071162	cellular_component	CMG complex
6	0000727	biological_process	double-strand break repair via break-induced replication
6	0003677	molecular_function	DNA binding
6	0003678	molecular_function	DNA helicase activity
6	0003688	molecular_function	DNA replication origin binding
6	0003697	molecular_function	single-stranded DNA binding
6	0004386	molecular_function	helicase activity
6	0005515	molecular_function	protein binding
6	0005524	molecular_function	ATP binding
6	0005634	cellular_component	nucleus
6	0005654	cellular_component	nucleoplasm
6	0005656	cellular_component	nuclear pre-replicative complex
6	0005737	cellular_component	cytoplasm
6	0006260	biological_process	DNA replication
6	0006267	biological_process	pre-replicative complex assembly involved in nuclear cell cycle DNA replication
6	0006268	biological_process	DNA unwinding involved in DNA replication
6	0006270	biological_process	DNA replication initiation
6	0006271	biological_process	DNA strand elongation involved in DNA replication
6	0006279	biological_process	premeiotic DNA replication
6	0008270	molecular_function	zinc ion binding
6	0009378	molecular_function	four-way junction helicase activity
6	0016787	molecular_function	hydrolase activity
6	0016887	molecular_function	ATP hydrolysis activity
6	0017116	molecular_function	single-stranded DNA helicase activity
6	0031261	cellular_component	DNA replication preinitiation complex
6	0031298	cellular_component	replication fork protection complex
6	0032508	biological_process	DNA duplex unwinding
6	0042555	cellular_component	MCM complex
6	0043138	molecular_function	3'-5' DNA helicase activity
6	0043596	cellular_component	nuclear replication fork
6	0071162	cellular_component	CMG complex
6	0097373	cellular_component	MCM core complex
6	1902969	biological_process	mitotic DNA replication
6	1990518	molecular_function	single-stranded 3'-5' DNA helicase activity
7	0000727	biological_process	double-strand break repair via break-induced replication
7	0000781	cellular_component	chromosome, telomeric region
7	0003677	molecular_function	DNA binding
7	0003678	molecular_function	DNA helicase activity
7	0003682	molecular_function	chromatin binding
7	0003688	molecular_function	DNA replication origin binding
7	0003697	molecular_function	single-stranded DNA binding
7	0004386	molecular_function	helicase activity
7	0005515	molecular_function	protein binding
7	0005524	molecular_function	ATP binding
7	0005634	cellular_component	nucleus
7	0005654	cellular_component	nucleoplasm
7	0005656	cellular_component	nuclear pre-replicative complex
7	0005737	cellular_component	cytoplasm
7	0006260	biological_process	DNA replication
7	0006267	biological_process	pre-replicative complex assembly involved in nuclear cell cycle DNA replication
7	0006268	biological_process	DNA unwinding involved in DNA replication
7	0006270	biological_process	DNA replication initiation
7	0006271	biological_process	DNA strand elongation involved in DNA replication
7	0006279	biological_process	premeiotic DNA replication
7	0008270	molecular_function	zinc ion binding
7	0009378	molecular_function	four-way junction helicase activity
7	0016787	molecular_function	hydrolase activity
7	0016887	molecular_function	ATP hydrolysis activity
7	0017116	molecular_function	single-stranded DNA helicase activity
7	0030466	biological_process	silent mating-type cassette heterochromatin formation
7	0031261	cellular_component	DNA replication preinitiation complex
7	0031298	cellular_component	replication fork protection complex
7	0031509	biological_process	subtelomeric heterochromatin formation
7	0032508	biological_process	DNA duplex unwinding
7	0033260	biological_process	nuclear DNA replication
7	0042555	cellular_component	MCM complex
7	0043138	molecular_function	3'-5' DNA helicase activity
7	0043596	cellular_component	nuclear replication fork
7	0071162	cellular_component	CMG complex
7	0097373	cellular_component	MCM core complex
7	1904931	molecular_function	MCM complex binding
A	0000727	biological_process	double-strand break repair via break-induced replication
A	0000811	cellular_component	GINS complex
A	0003674	molecular_function	molecular_function
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0006260	biological_process	DNA replication
A	0006261	biological_process	DNA-templated DNA replication
A	0006268	biological_process	DNA unwinding involved in DNA replication
A	0031261	cellular_component	DNA replication preinitiation complex
A	0031298	cellular_component	replication fork protection complex
A	0043596	cellular_component	nuclear replication fork
A	0071162	cellular_component	CMG complex
A	1902983	biological_process	DNA strand elongation involved in mitotic DNA replication
B	0000228	cellular_component	nuclear chromosome
B	0000727	biological_process	double-strand break repair via break-induced replication
B	0000811	cellular_component	GINS complex
B	0003674	molecular_function	molecular_function
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0006260	biological_process	DNA replication
B	0006261	biological_process	DNA-templated DNA replication
B	0006268	biological_process	DNA unwinding involved in DNA replication
B	0031261	cellular_component	DNA replication preinitiation complex
B	0031298	cellular_component	replication fork protection complex
B	0032993	cellular_component	protein-DNA complex
B	0043596	cellular_component	nuclear replication fork
B	0071162	cellular_component	CMG complex
C	0000727	biological_process	double-strand break repair via break-induced replication
C	0000811	cellular_component	GINS complex
C	0003674	molecular_function	molecular_function
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0006260	biological_process	DNA replication
C	0006261	biological_process	DNA-templated DNA replication
C	0006268	biological_process	DNA unwinding involved in DNA replication
C	0031261	cellular_component	DNA replication preinitiation complex
C	0031298	cellular_component	replication fork protection complex
C	0043596	cellular_component	nuclear replication fork
C	0071162	cellular_component	CMG complex
C	1902975	biological_process	mitotic DNA replication initiation
D	0000727	biological_process	double-strand break repair via break-induced replication
D	0000811	cellular_component	GINS complex
D	0003674	molecular_function	molecular_function
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0006260	biological_process	DNA replication
D	0006261	biological_process	DNA-templated DNA replication
D	0006268	biological_process	DNA unwinding involved in DNA replication
D	0031261	cellular_component	DNA replication preinitiation complex
D	0031298	cellular_component	replication fork protection complex
D	0043596	cellular_component	nuclear replication fork
D	0071162	cellular_component	CMG complex
E	0000727	biological_process	double-strand break repair via break-induced replication
E	0003682	molecular_function	chromatin binding
E	0003688	molecular_function	DNA replication origin binding
E	0003697	molecular_function	single-stranded DNA binding
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0006260	biological_process	DNA replication
E	0006268	biological_process	DNA unwinding involved in DNA replication
E	0006270	biological_process	DNA replication initiation
E	0031261	cellular_component	DNA replication preinitiation complex
E	0031298	cellular_component	replication fork protection complex
E	0043596	cellular_component	nuclear replication fork
E	0071162	cellular_component	CMG complex
E	1902977	biological_process	mitotic DNA replication preinitiation complex assembly
F	0000228	cellular_component	nuclear chromosome
F	0000278	biological_process	mitotic cell cycle
F	0000727	biological_process	double-strand break repair via break-induced replication
F	0003682	molecular_function	chromatin binding
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0006260	biological_process	DNA replication
F	0006261	biological_process	DNA-templated DNA replication
F	0006270	biological_process	DNA replication initiation
F	0006281	biological_process	DNA repair
F	0006334	biological_process	nucleosome assembly
F	0007064	biological_process	mitotic sister chromatid cohesion
F	0031298	cellular_component	replication fork protection complex
F	0034085	biological_process	establishment of sister chromatid cohesion
F	0035361	cellular_component	Cul8-RING ubiquitin ligase complex
F	0042802	molecular_function	identical protein binding
F	0043229	cellular_component	intracellular organelle
F	0043596	cellular_component	nuclear replication fork
G	0000228	cellular_component	nuclear chromosome
G	0000278	biological_process	mitotic cell cycle
G	0000727	biological_process	double-strand break repair via break-induced replication
G	0003682	molecular_function	chromatin binding
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0006260	biological_process	DNA replication
G	0006261	biological_process	DNA-templated DNA replication
G	0006270	biological_process	DNA replication initiation
G	0006281	biological_process	DNA repair
G	0006334	biological_process	nucleosome assembly
G	0007064	biological_process	mitotic sister chromatid cohesion
G	0031298	cellular_component	replication fork protection complex
G	0034085	biological_process	establishment of sister chromatid cohesion
G	0035361	cellular_component	Cul8-RING ubiquitin ligase complex
G	0042802	molecular_function	identical protein binding
G	0043229	cellular_component	intracellular organelle
G	0043596	cellular_component	nuclear replication fork
H	0000228	cellular_component	nuclear chromosome
H	0000278	biological_process	mitotic cell cycle
H	0000727	biological_process	double-strand break repair via break-induced replication
H	0003682	molecular_function	chromatin binding
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0006260	biological_process	DNA replication
H	0006261	biological_process	DNA-templated DNA replication
H	0006270	biological_process	DNA replication initiation
H	0006281	biological_process	DNA repair
H	0006334	biological_process	nucleosome assembly
H	0007064	biological_process	mitotic sister chromatid cohesion
H	0031298	cellular_component	replication fork protection complex
H	0034085	biological_process	establishment of sister chromatid cohesion
H	0035361	cellular_component	Cul8-RING ubiquitin ligase complex
H	0042802	molecular_function	identical protein binding
H	0043229	cellular_component	intracellular organelle
H	0043596	cellular_component	nuclear replication fork
X	0000076	biological_process	DNA replication checkpoint signaling
X	0003674	molecular_function	molecular_function
X	0003677	molecular_function	DNA binding
X	0005515	molecular_function	protein binding
X	0005634	cellular_component	nucleus
X	0005829	cellular_component	cytosol
X	0006260	biological_process	DNA replication
X	0006281	biological_process	DNA repair
X	0007064	biological_process	mitotic sister chromatid cohesion
X	0008156	biological_process	negative regulation of DNA replication
X	0031297	biological_process	replication fork processing
X	0031298	cellular_component	replication fork protection complex
X	0043111	biological_process	replication fork arrest
X	0043570	biological_process	maintenance of DNA repeat elements
X	0043596	cellular_component	nuclear replication fork
X	0051321	biological_process	meiotic cell cycle
Y	0000076	biological_process	DNA replication checkpoint signaling
Y	0003674	molecular_function	molecular_function
Y	0003677	molecular_function	DNA binding
Y	0005515	molecular_function	protein binding
Y	0005634	cellular_component	nucleus
Y	0005737	cellular_component	cytoplasm
Y	0006281	biological_process	DNA repair
Y	0006974	biological_process	DNA damage response
Y	0007064	biological_process	mitotic sister chromatid cohesion
Y	0008156	biological_process	negative regulation of DNA replication
Y	0031297	biological_process	replication fork processing
Y	0031298	cellular_component	replication fork protection complex
Y	0034087	biological_process	establishment of mitotic sister chromatid cohesion
Y	0043111	biological_process	replication fork arrest
Y	0043570	biological_process	maintenance of DNA repeat elements
Y	0043596	cellular_component	nuclear replication fork
Y	0045132	biological_process	meiotic chromosome segregation
Y	0051321	biological_process	meiotic cell cycle

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue ZN 2 901

Chain	Residue
2	CYS341
2	CYS344
2	CYS364
2	CYS367

site_id	AC2
Number of Residues	19
Details	binding site for residue ANP 2 902

Chain	Residue
2	ALA548
2	LYS549
2	SER550
2	GLN551
2	ASN651
2	LEU695
2	VAL699
2	MG903
6	GLU657
6	GLN658
6	ARG708
6	VAL797
6	ARG798
6	GLU801
2	TYR506
2	HIS508
2	PRO545
2	GLY546
2	THR547

site_id	AC3
Number of Residues	2
Details	binding site for residue MG 2 903

Chain	Residue
2	SER550
2	ANP902

site_id	AC4
Number of Residues	17
Details	binding site for residue ANP 3 1500

Chain	Residue
3	TYR372
3	PRO411
3	SER412
3	THR413
3	ALA414
3	LYS415
3	SER416
3	GLN417
3	ASN517
3	VAL565
3	MG1501
5	GLU498
5	GLN499
5	ARG549
5	ILE650
5	ARG651
5	GLU654

site_id	AC5
Number of Residues	2
Details	binding site for residue MG 3 1501

Chain	Residue
3	SER416
3	ANP1500

site_id	AC6
Number of Residues	4
Details	binding site for residue ZN 4 1400

Chain	Residue
4	CYS349
4	CYS352
4	CYS371
4	CYS376

site_id	AC7
Number of Residues	18
Details	binding site for residue ANP 5 801

Chain	Residue
2	HIS531
2	GLU625
2	GLN626
2	ARG676
2	VAL807
2	ARG808
2	GLU811
5	SER377
5	PHE379
5	PRO418
5	GLY419
5	THR420
5	ALA421
5	LYS422
5	SER423
5	GLN424
5	ASN524
5	MG802

site_id	AC8
Number of Residues	3
Details	binding site for residue MG 5 802

Chain	Residue
2	ARG676
5	SER423
5	ANP801

site_id	AC9
Number of Residues	4
Details	binding site for residue ZN 5 803

Chain	Residue
5	CYS183
5	CYS186
5	CYS211
5	CYS236

site_id	AD1
Number of Residues	4
Details	binding site for residue ZN 6 1400

Chain	Residue
6	CYS311
6	CYS314
6	CYS333
6	CYS338

site_id	AD2
Number of Residues	4
Details	binding site for residue ZN 7 1400

Chain	Residue
7	CYS262
7	CYS265
7	CYS284
7	CYS289

Functional Information from PROSITE/UniProt

site_id	PS00847
Number of Residues	9
Details	MCM_1 MCM family signature. GVCCIDEFD

Chain	Residue	Details
4	GLY627-ASP635
6	GLY634-ASP642
5	GLY475-ASP483
2	GLY602-ASP610
3	GLY468-ASP476
7	GLY519-ASP527

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:18407956

Chain	Residue	Details
X	SER626
X	SER654
G	SER377
G	SER379
H	SER377
H	SER379
7	ARG593
7	ARG687

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:19779198

Chain	Residue	Details
X	SER1056
X	SER1058
H	SER398

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:17330950, ECO:0007744\|PubMed:18407956, ECO:0007744\|PubMed:19779198

Chain	Residue	Details
X	SER1213
F	THR411
G	THR401
G	THR411
H	THR401
H	THR411

site_id	SWS_FT_FI4
Number of Residues	3
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:18407956

Chain	Residue	Details
F	SER463
G	SER463
H	SER463

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:17330950

Chain	Residue	Details
3	THR868

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		Validation report (PDF)