6SB2
cryo-EM structure of mTORC1 bound to active RagA/C GTPases
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | mTOR,Serine/threonine-protein kinase mTOR,mTOR,Serine/threonine-protein kinase mTOR | polymer | 2549 | 287235.2 | 2 | UniProt (P42345) Pfam (PF11865) Pfam (PF02259) Pfam (PF23593) Pfam (PF08771) Pfam (PF00454) Pfam (PF02260) | Homo sapiens | FK506-binding protein 12-rapamycin complex-associated protein 1,FKBP12-rapamycin complex-associated protein,Mammalian target of rapamycin,mTOR,Mechanistic target of rapamycin,Rapamycin and FKBP12 target 1,Rapamycin target protein 1,FK506-binding protein 12-rapamycin complex-associated protein 1,FKBP12-rapamycin complex-associated protein,Mammalian target of rapamycin,mTOR,Mechanistic target of rapamycin,Rapamycin and FKBP12 target 1,Rapamycin target protein 1 |
| 2 | C, G (E, H) | Target of rapamycin complex subunit LST8 | polymer | 326 | 35910.1 | 2 | UniProt (Q9BVC4) Pfam (PF00400) | Homo sapiens (Human) | TORC subunit LST8,G protein beta subunit-like,Protein GbetaL,Mammalian lethal with SEC13 protein 8,mLST8 |
| 3 | D, H (C, I) | Ras-related GTP-binding protein A | polymer | 313 | 36600.2 | 2 | UniProt (Q7L523) Pfam (PF04670) | Homo sapiens (Human) | RagA,Adenovirus E3 14.7 kDa-interacting protein 1,FIP-1 |
| 4 | E, I (D, J) | Ras-related GTP-binding protein C | polymer | 399 | 44284.8 | 2 | UniProt (Q9HB90) Pfam (PF04670) | Homo sapiens (Human) | RagC,GTPase-interacting protein 2,TIB929 |
| 5 | F, J (Y, N) | Regulatory-associated protein of mTOR | polymer | 1335 | 149200.0 | 2 | UniProt (Q8N122) Pfam (PF14538) Pfam (PF02985) Pfam (PF00400) | Homo sapiens (Human) | Raptor,p150 target of rapamycin (TOR)-scaffold protein |
| 6 | K, M (C, I) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 2 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 7 | L, N (D, J) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) |
Sequence modifications
A, B: -5 - 59 (PDB: 6SB2)
A, B: 60 - 355 (UniProt: P42345)
A, B: 356 - 380 (PDB: 6SB2)
A, B: 381 - 2549 (UniProt: P42345)
C, I: 1 - 313 (UniProt: Q7L523)
D, J: 1 - 399 (UniProt: Q9HB90)
A, B: 60 - 355 (UniProt: P42345)
A, B: 356 - 380 (PDB: 6SB2)
A, B: 381 - 2549 (UniProt: P42345)
C, I: 1 - 313 (UniProt: Q7L523)
| PDB | External Database | Details |
|---|---|---|
| Leu 66 | Gln 66 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Asn 90 | Thr 90 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 1106460.6 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1932.8 | |
| All* | Total formula weight | 1108393.4 |
