6S2F

Cryo-EM structure of Ctf18-1-8 in complex with the catalytic domain of DNA polymerase epsilon (Class 2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase epsilon catalytic subunit A	polymer	1219	140397.3	1	UniProt (P21951) Pfam (PF03104) Pfam (PF22634) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	DNA polymerase II subunit A
2	D	Chromosome transmission fidelity protein 8	polymer	133	15189.7	1	UniProt (P38877) Pfam (PF09696) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
3	E	Chromosome transmission fidelity protein 18	polymer	33	3859.3	1	UniProt (P49956) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
4	B	Sister chromatid cohesion protein DCC1	polymer	380	44133.8	1	UniProt (P25559) Pfam (PF09724) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Defective in sister chromatid cohesion protein 1
5	A	IRON/SULFUR CLUSTER	non-polymer		351.6	1	Chemie (SF4)

Sequence modifications

A: 1 - 1192 (UniProt: P21951)

PDB	External Database	Details
Met -26	-	initiating methionine
Lys -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
Ser -18	-	expression tag
Ala -17	-	expression tag
Gly -16	-	expression tag
Leu -15	-	expression tag
Glu -14	-	expression tag
Val -13	-	expression tag
Leu -12	-	expression tag
Phe -11	-	expression tag
Gln -10	-	expression tag
Gly -9	-	expression tag
Pro -8	-	expression tag
Gly -7	-	expression tag
Thr -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
Glu -3	-	expression tag
Phe -2	-	expression tag
Glu -1	-	expression tag
Leu 0	-	expression tag
Ala 290	Asp 290	conflict
Ala 292	Glu 292	conflict

E: 713 - 741 (UniProt: P49956)

PDB	External Database	Details
Gly 709	-	expression tag
Ala 710	-	expression tag
Met 711	-	expression tag
Gly 712	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	203580.1
Non-Polymers*	Number of molecules	1
	Total formula weight	351.6
All*	Total formula weight	203931.7

*Water molecules are not included.