6S1C

P3221 crystal form of the Ctf18-1-8/Pol2(1-528) complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, E)	DNA polymerase epsilon catalytic subunit A	polymer	549	62516.3	2	UniProt (P21951) Pfam (PF03104)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	DNA polymerase II subunit A
2	C, F (B, F)	Sister chromatid cohesion protein DCC1	polymer	380	44133.8	2	UniProt (P25559) Pfam (PF09724)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Defective in sister chromatid cohesion protein 1
3	D, G (C, G)	Chromosome transmission fidelity protein 8	polymer	133	15189.7	2	UniProt (P38877) Pfam (PF09696)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
4	E, H (D, H)	Chromosome transmission fidelity protein 18	polymer	33	3859.3	2	UniProt (P49956)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Sequence modifications

A, E: 1 - 524 (UniProt: P21951)

PDB	External Database	Details
Met -24	-	initiating methionine
Lys -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ala -15	-	expression tag
Gly -14	-	expression tag
Leu -13	-	expression tag
Glu -12	-	expression tag
Val -11	-	expression tag
Leu -10	-	expression tag
Phe -9	-	expression tag
Gln -8	-	expression tag
Gly -7	-	expression tag
Pro -6	-	expression tag
Gly -5	-	expression tag
Thr -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag
Ala 290	Asp 290	engineered mutation
Ala 292	Glu 292	engineered mutation

D, H: 713 - 741 (UniProt: P49956)

PDB	External Database	Details
Gly 709	-	expression tag
Ala 710	-	expression tag
Met 711	-	expression tag
Gly 712	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	251398.1
All*	Total formula weight	251398.1

*Water molecules are not included.