6S1C

P3221 crystal form of the Ctf18-1-8/Pol2(1-528) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0332
Spacegroup name	P 32 2 1
Unit cell lengths	126.334, 126.334, 378.427
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.970 - 6.100
R-factor	0.28
Rwork	0.275
R-free	0.33260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m8o 5OKC and 5OKI
RMSD bond length	0.003
RMSD bond angle	0.739
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.970	6.820
High resolution limit [Å]	6.100	6.100
Number of reflections	8632	2471
<I/σ(I)>	9.2	0.8
Completeness [%]	97.2	100
Redundancy	9.5	10
CC(1/2)	0.998	0.362

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M NaCl, 14% PEG 20000, 0.1 M HEPES pH 7.0