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6S0S

The crystal structure of kanamycin B dioxygenase (KanJ) from Streptomyces kanamyceticus in complex with nickel, ribostamycin B and 2-oxoglutarate

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A, B, C, D, E...
(A, B, C, D, E...)
Kanamycin B dioxygenasepolymer28831841.16UniProt (Q6L732)
Pfam (PF05721)
Streptomyces kanamyceticusKanamycin biosynthesis protein J
2G, J, N, R, U...
(A, B, C, D, E...)
NICKEL (II) IONnon-polymer58.76Chemie (NI)
3H, K, O, S, V...
(A, B, C, D, E...)
2-OXOGLUTARIC ACIDnon-polymer146.16Chemie (AKG)
4AA, I, L, P, T...
(F, A, B, C, D...)
RIBOSTAMYCINnon-polymer454.56Chemie (RIO)
5M, Q, X
(B, C, E)
CHLORIDE IONnon-polymer35.53Chemie (CL)
6BA, CA, DA, EA, FA...
(A, B, C, D, E...)
waterwater18.0462Chemie (HOH)
Sequence modifications
A, B, C, D, E, F: 1 - 285 (UniProt: Q6L732)
PDBExternal DatabaseDetails
Ser -2-expression tag
Asn -1-expression tag
Ala 0-expression tag
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains6
Total formula weight191046.6
Non-Polymers*Number of molecules21
Total formula weight4061.9
All*Total formula weight195108.5
*Water molecules are not included.

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PDB entries from 2025-12-17

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