6S0S

The crystal structure of kanamycin B dioxygenase (KanJ) from Streptomyces kanamyceticus in complex with nickel, ribostamycin B and 2-oxoglutarate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0016491	molecular_function	oxidoreductase activity
A	0016706	molecular_function	2-oxoglutarate-dependent dioxygenase activity
A	0017000	biological_process	antibiotic biosynthetic process
A	1901133	biological_process	kanamycin biosynthetic process
B	0016491	molecular_function	oxidoreductase activity
B	0016706	molecular_function	2-oxoglutarate-dependent dioxygenase activity
B	0017000	biological_process	antibiotic biosynthetic process
B	1901133	biological_process	kanamycin biosynthetic process
C	0016491	molecular_function	oxidoreductase activity
C	0016706	molecular_function	2-oxoglutarate-dependent dioxygenase activity
C	0017000	biological_process	antibiotic biosynthetic process
C	1901133	biological_process	kanamycin biosynthetic process
D	0016491	molecular_function	oxidoreductase activity
D	0016706	molecular_function	2-oxoglutarate-dependent dioxygenase activity
D	0017000	biological_process	antibiotic biosynthetic process
D	1901133	biological_process	kanamycin biosynthetic process
E	0016491	molecular_function	oxidoreductase activity
E	0016706	molecular_function	2-oxoglutarate-dependent dioxygenase activity
E	0017000	biological_process	antibiotic biosynthetic process
E	1901133	biological_process	kanamycin biosynthetic process
F	0016491	molecular_function	oxidoreductase activity
F	0016706	molecular_function	2-oxoglutarate-dependent dioxygenase activity
F	0017000	biological_process	antibiotic biosynthetic process
F	1901133	biological_process	kanamycin biosynthetic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue NI A 301

Chain	Residue
A	HIS132
A	ASP134
A	HIS219
A	AKG302
A	HOH401

---

site_id	AC2
Number of Residues	14
Details	binding site for residue AKG A 302

Chain	Residue
A	ASP134
A	THR165
A	HIS219
A	GLY221
A	ARG230
A	NI301
A	RIO303
A	HOH401
A	HOH412
A	HOH418
A	ASN73
A	ASN120
A	GLN129
A	HIS132

---

site_id	AC3
Number of Residues	19
Details	binding site for residue RIO A 303

Chain	Residue
A	ASN73
A	PHE74
A	GLN80
A	VAL116
A	SER118
A	ASN120
A	HIS132
A	LEU133
A	ASP134
A	GLU135
A	ARG183
A	ALA234
A	AKG302
A	HOH405
A	HOH409
A	HOH428
A	HOH432
A	HOH442
A	HOH444

---

site_id	AC4
Number of Residues	5
Details	binding site for residue NI B 301

Chain	Residue
B	HIS132
B	ASP134
B	HIS219
B	AKG302
B	HOH402

---

site_id	AC5
Number of Residues	13
Details	binding site for residue AKG B 302

Chain	Residue
B	ASN73
B	ASN120
B	GLN129
B	HIS132
B	ASP134
B	THR165
B	HIS219
B	ARG230
B	NI301
B	RIO303
B	HOH402
B	HOH439
B	HOH442

---

site_id	AC6
Number of Residues	17
Details	binding site for residue RIO B 303

Chain	Residue
B	ASN73
B	PHE74
B	GLN80
B	VAL116
B	SER118
B	ASN120
B	LEU133
B	ASP134
B	GLU135
B	ARG183
B	ARG213
B	ALA234
B	AKG302
B	HOH406
B	HOH436
B	HOH460
B	HOH493

---

site_id	AC7
Number of Residues	2
Details	binding site for residue CL B 304

Chain	Residue
B	PRO92
B	ASP93

---

site_id	AC8
Number of Residues	5
Details	binding site for residue NI C 301

Chain	Residue
C	HIS132
C	ASP134
C	HIS219
C	AKG302
C	HOH405

---

site_id	AC9
Number of Residues	14
Details	binding site for residue AKG C 302

Chain	Residue
C	ASN73
C	ASN120
C	GLN129
C	HIS132
C	ASP134
C	THR165
C	HIS219
C	GLY221
C	ARG230
C	NI301
C	RIO303
C	HOH405
C	HOH413
C	HOH417

---

site_id	AD1
Number of Residues	17
Details	binding site for residue RIO C 303

Chain	Residue
C	ASN120
C	LEU133
C	ASP134
C	GLU135
C	CYS150
C	ARG183
C	ALA234
C	AKG302
C	HOH411
C	HOH422
C	HOH437
C	HOH452
C	ASN73
C	PHE74
C	GLN80
C	VAL116
C	SER118

---

site_id	AD2
Number of Residues	3
Details	binding site for residue CL C 304

Chain	Residue
C	LEU187
C	PRO188
C	ALA189

---

site_id	AD3
Number of Residues	5
Details	binding site for residue NI D 301

Chain	Residue
D	HIS132
D	ASP134
D	HIS219
D	AKG302
D	HOH403

---

site_id	AD4
Number of Residues	13
Details	binding site for residue AKG D 302

Chain	Residue
D	ASN73
D	ASN120
D	GLN129
D	HIS132
D	ASP134
D	THR165
D	HIS219
D	ARG230
D	NI301
D	RIO303
D	HOH403
D	HOH415
D	HOH435

---

site_id	AD5
Number of Residues	16
Details	binding site for residue RIO D 303

Chain	Residue
D	ASN73
D	PHE74
D	GLN80
D	VAL116
D	SER118
D	ASN120
D	LEU133
D	ASP134
D	GLU135
D	CYS150
D	ARG183
D	ALA234
D	AKG302
D	HOH402
D	HOH452
D	HOH474

---

site_id	AD6
Number of Residues	5
Details	binding site for residue NI E 301

Chain	Residue
E	HIS132
E	ASP134
E	HIS219
E	AKG302
E	HOH401

---

site_id	AD7
Number of Residues	14
Details	binding site for residue AKG E 302

Chain	Residue
E	ASN73
E	ASN120
E	GLN129
E	HIS132
E	ASP134
E	THR165
E	HIS219
E	GLY221
E	ARG230
E	NI301
E	RIO303
E	HOH401
E	HOH404
E	HOH406

---

site_id	AD8
Number of Residues	14
Details	binding site for residue RIO E 303

Chain	Residue
E	ASN73
E	PHE74
E	VAL116
E	SER118
E	ASN120
E	HIS132
E	LEU133
E	ASP134
E	GLU135
E	ARG183
E	ARG213
E	ALA234
E	AKG302
E	HOH402

---

site_id	AD9
Number of Residues	3
Details	binding site for residue CL E 304

Chain	Residue
B	PRO188
B	ALA189
E	GLY77

---

site_id	AE1
Number of Residues	5
Details	binding site for residue NI F 301

Chain	Residue
F	HIS132
F	ASP134
F	HIS219
F	AKG302
F	HOH402

---

site_id	AE2
Number of Residues	13
Details	binding site for residue AKG F 302

Chain	Residue
F	ASN73
F	ASN120
F	GLN129
F	HIS132
F	ASP134
F	THR165
F	HIS219
F	ARG230
F	NI301
F	RIO303
F	HOH402
F	HOH413
F	HOH444

---

site_id	AE3
Number of Residues	17
Details	binding site for residue RIO F 303

Chain	Residue
F	ASN73
F	PHE74
F	GLN80
F	VAL116
F	ASN120
F	LEU133
F	ASP134
F	GLU135
F	ARG183
F	ARG213
F	AKG302
F	HOH405
F	HOH419
F	HOH442
F	HOH450
F	HOH455
F	HOH486

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