6ROJ
Cryo-EM structure of the activated Drs2p-Cdc50p
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Probable phospholipid-transporting ATPase DRS2,Oxaloacetate decarboxylase alpha chain | polymer | 1465 | 164240.4 | 1 | UniProt (P39524) UniProt (P13187) Pfam (PF16209) Pfam (PF00122) Pfam (PF13246) Pfam (PF16212) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 2 | B (C) | Cell division control protein 50 | polymer | 413 | 47371.8 | 1 | UniProt (P25656) Pfam (PF03381) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 3 | C, E (B, E) | beta-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 2 | In PDB GlyTouCan (G31886NL) | |||
| 4 | D (D) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 5 | F (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 6 | G (A) | (2R)-1-{[(R)-hydroxy{[(1R,2R,3R,4R,5S,6R)-2,3,5,6-tetrahydroxy-4-(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}-3-(octadecanoyloxy)propan-2-yl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate | non-polymer | 967.1 | 1 | Chemie (2Y5) | |||
| 7 | H (A) | water | water | 18.0 | 2 | Chemie (HOH) |
Sequence modifications
A: 1 - 1361 (UniProt: P39524)
A: 1380 - 1465 (UniProt: P13187)
C: 1 - 391 (UniProt: P25656)
| PDB | External Database | Details |
|---|---|---|
| Ser 1247 | - | insertion |
| Leu 1248 | - | insertion |
| Val 1249 | - | insertion |
| Pro 1250 | - | insertion |
| Arg 1251 | - | insertion |
| Gly 1252 | - | insertion |
| Gly 1362 | - | linker |
| Gly 1363 | - | linker |
| Gly 1364 | - | linker |
| Gly 1365 | - | linker |
| Leu 1366 | - | linker |
| Val 1367 | - | linker |
| Pro 1368 | - | linker |
| Arg 1369 | - | linker |
| Gly 1370 | - | linker |
| Ser 1371 | - | linker |
| Gly 1372 | - | linker |
| Gly 1373 | - | linker |
| Thr 1374 | - | linker |
| Ala 1375 | - | linker |
| Ala 1376 | - | linker |
| Ala 1377 | - | linker |
| Pro 1378 | - | linker |
| Gly 1379 | - | linker |
C: 1 - 391 (UniProt: P25656)
| PDB | External Database | Details |
|---|---|---|
| Gly 392 | - | expression tag |
| Gly 393 | - | expression tag |
| Gly 394 | - | expression tag |
| Gly 395 | - | expression tag |
| Leu 396 | - | expression tag |
| Val 397 | - | expression tag |
| Pro 398 | - | expression tag |
| Arg 399 | - | expression tag |
| Gly 400 | - | expression tag |
| Ser 401 | - | expression tag |
| Gly 402 | - | expression tag |
| Gly 403 | - | expression tag |
| His 404 | - | expression tag |
| His 405 | - | expression tag |
| His 406 | - | expression tag |
| His 407 | - | expression tag |
| His 408 | - | expression tag |
| His 409 | - | expression tag |
| His 410 | - | expression tag |
| His 411 | - | expression tag |
| His 412 | - | expression tag |
| His 413 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 211612.2 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1921.8 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 991.4 | |
| All* | Total formula weight | 214525.3 |
