6RIR

Crystal structure of phosphorylated Rab8a in complex with the Rab-binding domain of RILPL2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ras-related protein Rab-8A	polymer	184	21129.2	2	UniProt (P61006) Pfam (PF00071)	Homo sapiens (Human)	Oncogene c-mel
2	C, D (D, C)	RILP-like protein 2	polymer	43	5296.0	2	UniProt (Q969X0) Pfam (PF11461)	Homo sapiens (Human)	Rab-interacting lysosomal protein-like 2,p40phox-binding protein
3	E, H (A, B)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466)
4	F, I (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG) PubChem (888)
5	G (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL) PubChem (753)
6	J, K, L, M (A, B, D, C)	water	water		18.0	353	Chemie (HOH)

Sequence modifications

A, B: 1 - 181 (UniProt: P61006)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Leu 67	Gln 67	engineered mutation

D, C: 129 - 165 (UniProt: Q969X0)

PDB	External Database	Details
His 123	-	expression tag
His 124	-	expression tag
His 125	-	expression tag
His 126	-	expression tag
His 127	-	expression tag
His 128	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	52850.4
Non-Polymers*	Number of molecules	5
	Total formula weight	1187.1
All*	Total formula weight	54037.4

*Water molecules are not included.