6RIR
Crystal structure of phosphorylated Rab8a in complex with the Rab-binding domain of RILPL2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-31 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.333, 71.509, 114.784 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.410 - 1.767 |
R-factor | 0.1805 |
Rwork | 0.179 |
R-free | 0.21050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lhw |
RMSD bond length | 0.007 |
RMSD bond angle | 0.912 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.410 | 1.830 |
High resolution limit [Å] | 1.767 | 1.767 |
Rmerge | 0.069 | 0.949 |
Rmeas | 0.075 | 1.036 |
Rpim | 0.029 | 0.410 |
Number of reflections | 49201 | 4719 |
<I/σ(I)> | 16.4 | 1.69 |
Completeness [%] | 99.5 | 96.33 |
Redundancy | 6.6 | 6.2 |
CC(1/2) | 0.999 | 0.649 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 8% PEG 8K 100mM Na HEPES |