6R8F

Cryo-EM structure of the Human BRISC-SHMT2 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Lys-63-specific deubiquitinase BRCC36	polymer	316	36119.9	2	UniProt (P46736) Pfam (PF01398) Pfam (PF18110) In PDB	Homo sapiens (Human)	BRCA1-A complex subunit BRCC36,BRCA1/BRCA2-containing complex subunit 3,BRCA1/BRCA2-containing complex subunit 36,BRISC complex subunit BRCC36
2	B, D	BRISC complex subunit Abraxas 2	polymer	267	31033.9	2	UniProt (Q15018) Pfam (PF21125) In PDB	Homo sapiens (Human)	Abraxas brother protein 1,Protein FAM175B
3	K, L	Serine hydroxymethyltransferase, mitochondrial	polymer	504	56097.9	2	UniProt (P34897) Pfam (PF00464) In PDB	Homo sapiens (Human)	SHMT,Glycine hydroxymethyltransferase,Serine methylase
4	E, G	BRISC and BRCA1-A complex member 2,BRCC45 (BRE, BRISC and BRCA1-A complex member 2)	polymer	217	22026.7	2	UniProt (Q9NXR7) Pfam (PF06113) In PDB	Homo sapiens (Human)	BRCA1-A complex subunit BRE,BRCA1/BRCA2-containing complex subunit 45,Brain and reproductive organ-expressed protein
5	A, C	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

K, L: 1 - 504 (UniProt: P34897)

PDB	External Database	Details
Thr 285	Ala 285	engineered mutation

E, G: 1 - 131 (UniProt: Q9NXR7)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag

E, G: 132 - 255 (PDB: 6R8F)

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	290557.0
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	290687.8

*Water molecules are not included.