6R8F

Cryo-EM structure of the Human BRISC-SHMT2 complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000151	cellular_component	ubiquitin ligase complex
A	0000152	cellular_component	nuclear ubiquitin ligase complex
A	0000922	cellular_component	spindle pole
A	0004843	molecular_function	cysteine-type deubiquitinase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006281	biological_process	DNA repair
A	0006282	biological_process	regulation of DNA repair
A	0006302	biological_process	double-strand break repair
A	0006325	biological_process	chromatin organization
A	0006508	biological_process	proteolysis
A	0006974	biological_process	DNA damage response
A	0007095	biological_process	mitotic G2 DNA damage checkpoint signaling
A	0008233	molecular_function	peptidase activity
A	0008237	molecular_function	metallopeptidase activity
A	0010165	biological_process	response to X-ray
A	0010212	biological_process	response to ionizing radiation
A	0016579	biological_process	protein deubiquitination
A	0016787	molecular_function	hydrolase activity
A	0030234	molecular_function	enzyme regulator activity
A	0031593	molecular_function	polyubiquitin modification-dependent protein binding
A	0034516	biological_process	response to vitamin B6
A	0044818	biological_process	mitotic G2/M transition checkpoint
A	0045739	biological_process	positive regulation of DNA repair
A	0046872	molecular_function	metal ion binding
A	0051301	biological_process	cell division
A	0061578	molecular_function	K63-linked deubiquitinase activity
A	0070531	cellular_component	BRCA1-A complex
A	0070536	biological_process	protein K63-linked deubiquitination
A	0070552	cellular_component	BRISC complex
A	0071479	biological_process	cellular response to ionizing radiation
A	0140492	molecular_function	metal-dependent deubiquitinase activity
A	0140861	biological_process	DNA repair-dependent chromatin remodeling
A	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
A	2000001	biological_process	regulation of DNA damage checkpoint
C	0000151	cellular_component	ubiquitin ligase complex
C	0000152	cellular_component	nuclear ubiquitin ligase complex
C	0000922	cellular_component	spindle pole
C	0004843	molecular_function	cysteine-type deubiquitinase activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006281	biological_process	DNA repair
C	0006282	biological_process	regulation of DNA repair
C	0006302	biological_process	double-strand break repair
C	0006325	biological_process	chromatin organization
C	0006508	biological_process	proteolysis
C	0006974	biological_process	DNA damage response
C	0007095	biological_process	mitotic G2 DNA damage checkpoint signaling
C	0008233	molecular_function	peptidase activity
C	0008237	molecular_function	metallopeptidase activity
C	0010165	biological_process	response to X-ray
C	0010212	biological_process	response to ionizing radiation
C	0016579	biological_process	protein deubiquitination
C	0016787	molecular_function	hydrolase activity
C	0030234	molecular_function	enzyme regulator activity
C	0031593	molecular_function	polyubiquitin modification-dependent protein binding
C	0034516	biological_process	response to vitamin B6
C	0044818	biological_process	mitotic G2/M transition checkpoint
C	0045739	biological_process	positive regulation of DNA repair
C	0046872	molecular_function	metal ion binding
C	0051301	biological_process	cell division
C	0061578	molecular_function	K63-linked deubiquitinase activity
C	0070531	cellular_component	BRCA1-A complex
C	0070536	biological_process	protein K63-linked deubiquitination
C	0070552	cellular_component	BRISC complex
C	0071479	biological_process	cellular response to ionizing radiation
C	0140492	molecular_function	metal-dependent deubiquitinase activity
C	0140861	biological_process	DNA repair-dependent chromatin remodeling
C	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
C	2000001	biological_process	regulation of DNA damage checkpoint
E	0070531	cellular_component	BRCA1-A complex
E	0070552	cellular_component	BRISC complex
G	0070531	cellular_component	BRCA1-A complex
G	0070552	cellular_component	BRISC complex
K	0002082	biological_process	regulation of oxidative phosphorylation
K	0003682	molecular_function	chromatin binding
K	0004372	molecular_function	glycine hydroxymethyltransferase activity
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005737	cellular_component	cytoplasm
K	0005739	cellular_component	mitochondrion
K	0005743	cellular_component	mitochondrial inner membrane
K	0005759	cellular_component	mitochondrial matrix
K	0006417	biological_process	regulation of translation
K	0006544	biological_process	glycine metabolic process
K	0006545	biological_process	glycine biosynthetic process
K	0006563	biological_process	L-serine metabolic process
K	0006564	biological_process	L-serine biosynthetic process
K	0006730	biological_process	one-carbon metabolic process
K	0008284	biological_process	positive regulation of cell population proliferation
K	0008732	molecular_function	L-allo-threonine aldolase activity
K	0015943	biological_process	formate biosynthetic process
K	0016597	molecular_function	amino acid binding
K	0016740	molecular_function	transferase activity
K	0019264	biological_process	glycine biosynthetic process from serine
K	0030170	molecular_function	pyridoxal phosphate binding
K	0034340	biological_process	response to type I interferon
K	0035999	biological_process	tetrahydrofolate interconversion
K	0042645	cellular_component	mitochondrial nucleoid
K	0042802	molecular_function	identical protein binding
K	0046653	biological_process	tetrahydrofolate metabolic process
K	0051262	biological_process	protein tetramerization
K	0051289	biological_process	protein homotetramerization
K	0070013	cellular_component	intracellular organelle lumen
K	0070062	cellular_component	extracellular exosome
K	0070129	biological_process	regulation of mitochondrial translation
K	0070536	biological_process	protein K63-linked deubiquitination
K	0070552	cellular_component	BRISC complex
K	0120567	molecular_function	hydroxytrimethyllysine aldolase activity
K	1903715	biological_process	regulation of aerobic respiration
L	0002082	biological_process	regulation of oxidative phosphorylation
L	0003682	molecular_function	chromatin binding
L	0004372	molecular_function	glycine hydroxymethyltransferase activity
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005737	cellular_component	cytoplasm
L	0005739	cellular_component	mitochondrion
L	0005743	cellular_component	mitochondrial inner membrane
L	0005759	cellular_component	mitochondrial matrix
L	0006417	biological_process	regulation of translation
L	0006544	biological_process	glycine metabolic process
L	0006545	biological_process	glycine biosynthetic process
L	0006563	biological_process	L-serine metabolic process
L	0006564	biological_process	L-serine biosynthetic process
L	0006730	biological_process	one-carbon metabolic process
L	0008284	biological_process	positive regulation of cell population proliferation
L	0008732	molecular_function	L-allo-threonine aldolase activity
L	0015943	biological_process	formate biosynthetic process
L	0016597	molecular_function	amino acid binding
L	0016740	molecular_function	transferase activity
L	0019264	biological_process	glycine biosynthetic process from serine
L	0030170	molecular_function	pyridoxal phosphate binding
L	0034340	biological_process	response to type I interferon
L	0035999	biological_process	tetrahydrofolate interconversion
L	0042645	cellular_component	mitochondrial nucleoid
L	0042802	molecular_function	identical protein binding
L	0046653	biological_process	tetrahydrofolate metabolic process
L	0051262	biological_process	protein tetramerization
L	0051289	biological_process	protein homotetramerization
L	0070013	cellular_component	intracellular organelle lumen
L	0070062	cellular_component	extracellular exosome
L	0070129	biological_process	regulation of mitochondrial translation
L	0070536	biological_process	protein K63-linked deubiquitination
L	0070552	cellular_component	BRISC complex
L	0120567	molecular_function	hydroxytrimethyllysine aldolase activity
L	1903715	biological_process	regulation of aerobic respiration

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	binding site for residue ZN A 401

Chain	Residue
A	HIS122
A	HIS124
A	ASP135

---

site_id	AC2
Number of Residues	3
Details	binding site for residue ZN C 401

Chain	Residue
C	HIS122
C	HIS124
C	ASP135

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	26
Details	Motif: {"description":"JAMM motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01182","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01182","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	14
Details	Region: {"description":"Important for interaction with SHMT2","evidences":[{"source":"PubMed","id":"24075985","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	42
Details	Region: {"description":"Important for interaction with BBRC36 and other subunits of the BRISC complex","evidences":[{"source":"PubMed","id":"26344097","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	12
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"PubMed","id":"29180469","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---