6R5P

The crystal structure of Glycoside Hydrolase BglX inactive mutant D286N from P. aeruginosa in complex with glucose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, A)	Periplasmic beta-glucosidase	polymer	765	83423.4	2	UniProt (Q9I311) Pfam (PF00933) Pfam (PF01915) Pfam (PF14310)	Pseudomonas aeruginosa PAO1
2	C, E (B, A)	beta-D-glucopyranose	non-polymer		180.2	2	Chemie (BGC)
3	D, F (B, A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	G, H (B, A)	water	water		18.0	188	Chemie (HOH)

Sequence modifications

B, A: 21 - 764 (UniProt: Q9I311)

PDB	External Database	Details
Met 0	-	initiating methionine
Gly 1	-	expression tag
Ser 2	-	expression tag
Ser 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
Ser 10	-	expression tag
Ser 11	-	expression tag
Gly 12	-	expression tag
Leu 13	-	expression tag
Val 14	-	expression tag
Pro 15	-	expression tag
Arg 16	-	expression tag
Gly 17	-	expression tag
Ser 18	-	expression tag
His 19	-	expression tag
Met 20	-	expression tag
Asn 286	Asp 286	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	166846.9
Non-Polymers*	Number of molecules	4
	Total formula weight	408.9
All*	Total formula weight	167255.8

*Water molecules are not included.