6R5P
The crystal structure of Glycoside Hydrolase BglX inactive mutant D286N from P. aeruginosa in complex with glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979160 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.713, 73.624, 81.509 |
| Unit cell angles | 65.38, 73.41, 69.89 |
Refinement procedure
| Resolution | 45.160 - 2.850 |
| R-factor | 0.17543 |
| Rwork | 0.173 |
| R-free | 0.22278 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tf0 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.298 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.160 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rpim | 0.130 | 0.460 |
| Number of reflections | 31258 | 4491 |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 96.8 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 100 mM Bis Tris, pH 5.5, 17% PEG 10000 and 100 mM ammonium acetate |
